FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755830

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755830
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences780448
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24300.041.2317161
GGTATCA10600.038.6532331
GTGGTAT3100.035.541271
TCAACGC28700.034.829954
ATCAACG29150.034.1875653
CAACGCA29250.034.172845
AACGCAG29850.033.8311656
TATCAAC29500.033.801452
ACGCAGA30900.032.57047
AGAGTAC32400.031.27454211
CAGAGTA33000.030.8110
CGCAGAG32900.030.694848
GCAGAGT34950.028.9927089
TTACCGC500.006644355628.47153366
ATTTCGC500.006644355628.47153366
GAGTACG28700.028.00579312
AGTACGG28750.027.83761413
CGAGCGA656.8605144E-427.61478667
ACTGACG1857.812323E-726.22867270
ACGGGTT1457.385097E-1026.06119216
Produced by FastQC (version 0.11.5)