FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755837

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755837
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences577660
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA22710.3931378319426652No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21400.3704601322577295No Hit
GTAATATACGGCCGTGTCCGCAACGGTCACAGAGTTCAGATTCAGGGAGAACTGGTTGTTGGACGTGTCTGCCGA16200.2804417823633279No Hit
GTCTCTGGTGGCTCCATCAGTGGTTACTATTGGGGCTGGATCCGGCAGCC15660.271093722951217No Hit
GTATATTACTGTCAGCATTATGGTGGTTCACCTCCTCTCACTTTCGGCGG14420.2496278087456289No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13810.23906796385417028No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12950.22418031367932695No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12820.2219298549319669No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12820.2219298549319669No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12780.22123740608662534No Hit
GTGTTAGTAGCATTCACCTGGCCTGGTACCAGCAGAGACCTGGCCATGCTCCCAGGCTCCTCATTCATAGTGCAT11890.20583041927777584No Hit
GTAATATACCGCAAAGTCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCGCTGCCA11860.20531108264376968No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11230.19440501332964027No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC11170.19336634006162795No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC10630.18401828064951703No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC10500.18176782190215698No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGTAGCATTCACCTGGCCTGGTACC10460.18107537305681543No Hit
GTCCTGGACCTCCTGTGCAAGAGCATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC10420.18038292421147387No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10290.17813246546411382No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9940.17207353806737527No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9910.1715542014333691No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9870.17086175258802758No Hit
GTCTCCAGGCTCCCTGTCTTTGTCTCCAGGGGATAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTAG9600.1661877228819721No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9390.16255236644392895No Hit
ATATTACTGTCAGCATTATGGTGGTTCACCTCCTCTCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAA9250.16012879548523354No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT9200.15926323442855658No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT9190.1590901222172212No Hit
CTCCAGGCTCCCTGTCTTTGTCTCCAGGGGATAGAGCCACCCTCTCCTGC9070.15701277568119654No Hit
GTATATTACTGTGCGAGACTCGATAGTGGAAACGATCGACACGGCTATTATTACTACTACCACGGTTTGGACGT9040.1564934390471904No Hit
GCATTATGGTGGTTCACCTCCTCTCACTTTCGGCGGAGGGACCAGGGTGG8900.15406986808849496No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8710.1507807360731226No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG8650.14974206280511027No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCCCTCCGCCGAAAGTGAGAGGAGGTGAACCACCATAATG8590.14870338953709794No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG8530.14766471626908562No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAGGACTTTGCGGTATATTACTGTCAGCATTATGGTGGTTCA8100.14022089118166395No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7950.13762420801163314No Hit
CCATAATGCTGACAGTAATATACCGCAAAGTCCTCAGGCTCCAGTCTGCT7850.13589308589827925No Hit
CTATTGGGGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGTTTGTCTATTACAATGGGAGAAC7800.13502752484160233No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA7790.13485441263026693No Hit
GGATAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTAGCATTCACCTGGCCTGGTACCAGCAGAGAC7670.13277706609424228No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG7640.13225772946023612No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT7630.13208461724890072No Hit
CAGTAATATACCGCAAAGTCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCGCTGCCA7550.13069971955821763No Hit
GTCTTTGTCTCCAGGGGATAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA7550.13069971955821763No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC7490.1296610462902053No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7370.12758369975418066No Hit
CTCCCAGGCTCCTCATTCATAGTGCATCCCGCAGGGCCACTGGCATCCCA7190.12446767995014367No Hit
GTGCAAGAGCATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTC7190.12446767995014367No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7180.1242945677388083No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG7110.12308278225946058No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT7060.12221722120278364No Hit
GTACCAGCAGAGACCTGGCCATGCTCCCAGGCTCCTCATTCATAGTGCATCCCGCAGGGCCACTGGCATCCCAGA6920.11979365024408822No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA6920.11979365024408822No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAGTTGTGTTGACGCAGTCTC6790.11754319149672818No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6780.11737007928539278No Hit
CATTCATAGTGCATCCCGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGCGGGTCTGGGACAGACTTCA6770.11719696707405741No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAGTTGTGTTGACGCAGTC6710.11615829380604507No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCC6670.11546584496070353No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC6640.11494650832669737No Hit
CTTTCTGAGAGTCCTGGACCTCCTGTGCAAGAGCATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCC6470.11200360073399576No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT6290.1088875809299588No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC6260.10836824429595264No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6190.10715645881660492No Hit
CCTCATTCATAGTGCATCCCGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGCGGGTCTGGGACAGACTT6160.10663712218259877No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT6110.10577156112592183No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG6080.10525222449191565No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTAGCATTCACCTG6020.10421355122390333No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT5990.10369421458989717No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA5980.10352110237856178No Hit
TTCATAGTGCATCCCGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGCGGGTCTGGGACAGACTTCACT5940.10282865353322024No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT5900.10213620468787869No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG5890.1019630924765433No Hit
CTATGAATGAGGAGCCTGGGAGCATGGCCAGGTCTCTGCTGGTACCAGGCCAGGTGAATGCTACTAACACTCTGA5870.10161686805387252No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA5830.10092441920853097No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA5780.10005885815185403No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACAACG200.00737198951.72370521
GTATCAA11000.045.1406821
GGTATCA3750.044.1375581
GTCGTAA350.001302464339.70067648
TATCAAC13200.037.6107252
ATGTGTA754.038884E-836.77493711
TCAACGC13950.035.3415034
ATCAACG14050.035.3353463
CAACGCA14150.034.8419765
AACGCAG14300.034.47656
TAGGCAA750.002919663233.6763570
CTGCCGA5350.033.05127369
CGCAGAG15300.032.2231378
AGAGTAC15650.031.72278611
CGTAAAC450.0044469630.9079650
ACGCAGA16000.030.8133727
GCAGAGT16000.030.8133729
CTGATAT450.00463427230.64843220
TACATGA450.004636234630.645782
TACACAT450.004636234630.645785
Produced by FastQC (version 0.11.5)