FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755843

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755843
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences675394
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29040.4299712464132048No Hit
GAGTTACATCACCCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAGCGAACTGTGGCTGCACCAT25860.3828876181902712No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22210.32884508894067754No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG21670.32084975584621717No Hit
CTCCTGATCTGTGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGATT21070.3119660524079278No Hit
GTGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGATTCAGTGGTAGTGGATCTGGGACAGATTTCACTCTCA20880.3091528796524695No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19360.28664749760880315No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18830.27880022623831424No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18200.2694723376281104No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18030.2669552883205951No Hit
GTGCAGCCACAGTTCGCTTGATTTCCACCTTGGTCCCCTGGCCGAACGTC17680.26177312798159297No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16910.25037237523578826No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16450.24356153593309982No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG15450.22875536353595088No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14260.21113601838334364No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14160.20965540114362877No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14070.20832284562788536No Hit
ATGTAACTCTGTTGACAGTAGTAAGATGCAATATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC13790.20417711735668367No Hit
ACATCACCCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAGCGA13460.1992910804656245No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13290.1967740311581092No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT13270.1964779077101662No Hit
GATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGTTTAGAT13050.19322054978279346No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT12930.1914438090951356No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC12110.17930274772947347No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC11790.1745647725623858No Hit
GGGTGATGTAACTCTGTTGACAGTAGTAAGATGCAATATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT11630.17219578497884197No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAATAT11500.1702709825672126No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11460.16967873567132669No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATTTCCACCTTGGTCC11270.16686556291586838No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAATATCTATCTAAACTGGTATCAGCAGAAA11140.164940760504239No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11110.16449657533232453No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11000.16286789636863816No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10970.1624237111967237No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10740.15901829154537944No Hit
GTAGTAAGATGCAATATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTACCA10640.15753767430566454No Hit
TCTTACTACTGTCAACAGAGTTACATCACCCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAGCGAA10250.15176326707077645No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10120.1498384646591471No Hit
ATGCAGCACAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGTTTAGATAGATATTAATGCT10040.1486539708673752No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10020.14835784741943223No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATATTGCATCTTACTACTGTCAACAGAGTTACATCACC9810.14524855121603095No Hit
CCCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAGCGAACTGTGGCTGCACCATCTGTCTTCATCTTCC9680.14332374880440157No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9680.14332374880440157No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9290.1375493415695135No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9240.13680903294965605No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT9230.13666097122568457No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9060.13414392191816923No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC8940.1323671812305114No Hit
ATCTAAACTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATC8560.12674083571959477No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8320.12318735434427905No Hit
GAGTGAAATCTGTCCCAGATCCACTACCACTGAATCTTGATGGGACCCCA8300.12289123089633608No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8130.12037418158882074No Hit
GTAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCT8030.11889356434910585No Hit
GTGGGGTCCCATCAAGATTCAGTGGTAGTGGATCTGGGACAGATTTCACT7880.1166726384895335No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7860.11637651504159054No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC7790.11534008297379011No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA7710.1141555891820182No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG7650.11326721883818927No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA7620.1128230336662748No Hit
CACCATCAGCAGTCTGCAACCTGAAGATATTGCATCTTACTACTGTCAACAGAGTTACATCACCCCGTGGACGTT7610.1126749719423033No Hit
GTGGTAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATATTGCATCTTACTA7490.11089823125464543No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7480.11075016953067393No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT7280.10778893505124416No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC7260.1074928116033012No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC7220.10690056470741523No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC7180.10630831781152927No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC7110.10527188574372885No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA7020.10393933022798543No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTACCACTGAATCTTG7010.10379126850401396No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT7000.10364320678004246No Hit
GTCTGCAACCTGAAGATATTGCATCTTACTACTGTCAACAGAGTTACATCACCCCGTGGACGTTCGGCCAGGGGA6950.10290289816018501No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCA6810.10083003402458417No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA6800.10068197230061267No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA200.007144968552.1354246
AATTGCG200.00733252151.79491835
TCGTATG350.001323185439.574440
TATTAGG350.001357919839.3663832
GTATCAA14000.038.6311231
GTGGTAT2000.034.4481351
TATCAAC16400.033.185382
ATCAACG16850.032.09473
CAACGCA17300.031.658085
TCAACGC17100.031.6254774
AACGCAG17450.031.3859486
TGACTAA450.00428178731.14870561
TAGGATA450.004657177330.6182984
GCGCTAT450.004657177330.6182986
AGAGTAC18550.029.52697211
ATATGAG703.890364E-529.5247863
TGCCCGG6950.029.38456570
CGTAAAC952.9407965E-729.33475350
ACGCAGA18750.029.2098547
CGCAGAG19100.028.6745978
Produced by FastQC (version 0.11.5)