FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755844

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755844
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences675394
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26560.3932519388682754No Hit
GAGTTACATCACCCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAGCGAACTGTGGCTGCACCAT24700.36571245820957843No Hit
CTCCTGATCTGTGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGATT22130.3276605951489057No Hit
GTGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGATTCAGTGGTAGTGGATCTGGGACAGATTTCACTCTCA22010.3258838544612478No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG21960.3251435458413904No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19560.2896087320882329No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18290.2708048931438538No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17280.2558506590227334No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17260.2555545355747904No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16630.2462266469645866No Hit
GTGCAGCCACAGTTCGCTTGATTTCCACCTTGGTCCCCTGGCCGAACGTC14990.22194452423326236No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG14980.22179646250929086No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14890.22046390699354745No Hit
ACATCACCCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAGCGA14160.20965540114362877No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14130.2092112159717143No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13590.2012158828772539No Hit
GGGTGATGTAACTCTGTTGACAGTAGTAAGATGCAATATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT13470.199439142189596No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT13330.19736627805399515No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAATAT13300.1969220928820807No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC13200.1954414756423658No Hit
GATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGTTTAGAT12960.19188799426705005No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT12870.19055543875130665No Hit
ATGTAACTCTGTTGACAGTAGTAAGATGCAATATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC12820.1898151301314492No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAATATCTATCTAAACTGGTATCAGCAGAAA12440.1841887846205326No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12230.18107948841713134No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12120.17945080945344494No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12030.17811825393770156No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11350.16805005670764028No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11100.16434851360835306No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC10710.15857410637346497No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10530.15590899534197816No Hit
TCTTACTACTGTCAACAGAGTTACATCACCCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAGCGAA10530.15590899534197816No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10140.15013458810709007No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATATTGCATCTTACTACTGTCAACAGAGTTACATCACC9830.14554467466397392No Hit
ATGCAGCACAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGTTTAGATAGATATTAATGCT9740.1442121191482305No Hit
GTGGGGTCCCATCAAGATTCAGTGGTAGTGGATCTGGGACAGATTTCACT9550.14139894639277223No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9500.14065863777291476No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATTTCCACCTTGGTCC9270.13725321812157054No Hit
CCCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAGCGAACTGTGGCTGCACCATCTGTCTTCATCTTCC9180.1359206626058271No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC9180.1359206626058271No Hit
GTAGTAAGATGCAATATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTACCA9150.13547647743391264No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9020.1335516750222833No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9010.1334036132983118No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8990.13310748985036883No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8960.13266330467845436No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT8820.13059044054285351No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA8540.1264447122716518No Hit
ATCTAAACTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATC8460.1252602184798799No Hit
GTGGTAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATATTGCATCTTACTA8450.1251121567559084No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8340.12348347779222203No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8000.1184493791771914No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA7790.11534008297379011No Hit
CACCATCAGCAGTCTGCAACCTGAAGATATTGCATCTTACTACTGTCAACAGAGTTACATCACCCCGTGGACGTT7780.11519202124981862No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT7570.11208272504641735No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7530.1114904781505314No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC7490.11089823125464543No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCA7360.10897342884301608No Hit
GTAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCT7310.10823312022315862No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7290.10793699677521566No Hit
GAGTGAAATCTGTCCCAGATCCACTACCACTGAATCTTGATGGGACCCCA7290.10793699677521566No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC7240.1071966881553582No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA7240.1071966881553582No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC7170.10616025608755777No Hit
GTCTGCAACCTGAAGATATTGCATCTTACTACTGTCAACAGAGTTACATCACCCCGTGGACGTTCGGCCAGGGGA7030.10408739195195693No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC7000.10364320678004246No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC6900.10216258954032759No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAATC150.002215836470.06882560
GTATCAA13050.044.944171
CGAACTA602.937977E-740.19775829
CACACCG650.001476458538.69708670
TATCAAC15650.037.6447682
ATCAACG16100.036.3489573
TCAACGC16250.036.209184
CAACGCA16500.035.452015
AACGCAG16650.035.3392876
GTGGTAT1600.034.5012631
AGAGTAC17900.032.8714611
ACGCAGA18550.031.9051257
CGCAGAG18950.031.2316658
GCAGAGT19700.029.8679759
AGTACGG17400.029.86094913
GAGTACG17750.029.46599812
TAAGACT953.2750904E-728.976269
CAGAGTA20450.028.77257310
TACGGGG11000.025.65247315
AAAGCGA951.065447E-525.3730125
Produced by FastQC (version 0.11.5)