FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences469731
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAACTGGCCTCTCACTTTCGGCGGCGGGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCAT30130.641430946648188No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACCA27560.586718781600533No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24730.52647153370759No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18930.40299660869731824No Hit
GTTATTATACTGTTGACAGTAATAAACTGCAAAATCCTCAGACTGCAGGCTGTCGATGGTGAGAGTGAACT18710.3983130770589976No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15630.3327436341225084No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15270.3250796732598019No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC15010.31954459041451383No Hit
GTCCCAGACCCACTGCCACTGAAGCTGGCTGGGATACCAGTGGCCCTAGT14030.2986815858438127No Hit
GTAATATACAGCCGTATCCTCGACTCTCAGGCTGTTCATTTGTAGATACA13960.29719137123161976No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13910.2961269322229106No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13500.28739853235149476No Hit
GTCTGGGACAGAGTTCACTCTCACCATCGACAGCCTGCAGTCTGAGGATT13350.2842052153253671No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT13290.282927888514916No Hit
CTCCTGCAGGGCCAGTCAGAGCGTCACCACCAACTTAGCCTGGTTCCAGCAGAAGCCTGACCAGGCTCCCAGGCT13130.2795216836870464No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGCGTCACCACCAACTTAGCCTGGTT12960.275902591057435No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12710.27058039601388884No Hit
GAGTTCACTCTCACCATCGACAGCCTGCAGTCTGAGGATTTTGCAGTTTATTACTGTCAACAGTATAATAACTGG12290.2616391083407312No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGCGTCACCAC12130.2582329035128616No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12020.2558911376937013No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11580.24652407441705998No Hit
CATTTATGGTGCATCCACTAGGGCCACTGGTATCCCAGCCAGCTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA11140.23715701114041865No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11070.23566679652822572No Hit
CTGGTATCCCAGCCAGCTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT10680.22736417226029365No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10680.22736417226029365No Hit
GTATATTACTGTGCGAGAGAATCCATTTCAGGAAATGGGTATGAGAAGTA10330.21991309919932897No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10140.21586823096623386No Hit
CCATAAATGAGGAGCCTGGGAGCCTGGTCAGGCTTCTGCTGGAACCAGGC10050.21395224075055724No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10000.21288780174184802No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9800.20863004570701102No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9740.20735271889655996No Hit
GTAATAAACTGCAAAATCCTCAGACTGCAGGCTGTCGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA9630.2050109530773996No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAAGCTGGCTGGGATACCA9510.20245629945649743No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9400.2001145336373371No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9000.1915990215676632No Hit
GGATACCAGTGGCCCTAGTGGATGCACCATAAATGAGGAGCCTGGGAGCCTGGTCAGGCTTCTGCTGGAACCAGG8920.18989591915372842No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8750.18627682652411698No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT8260.17584532423876645No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCT8200.17456799742831536No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8110.1726520072126387No Hit
CACTAGGGCCACTGGTATCCCAGCCAGCTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCACCATCGACAG7840.16690403656560882No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC7710.1641364951429648No Hit
CTGCTACTCTGGCTCCCAGAAACCACTGGAAGAGTAGTGATGACGCAGTC7590.16158184152206262No Hit
CAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAAC7570.16115606591857892No Hit
GTTTATTACTGTCAACAGTATAATAACTGGCCTCTCACTTTCGGCGGCGGGACCAAGGTGGACATCAAACGAA7570.16115606591857892No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7500.159665851306386No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7370.15689830988374198No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC7190.1530663294523887No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7160.15242766604716315No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7080.1507245636332284No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT6990.14880857341755177No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6980.14859568561580988No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACCACTGGAAGAGTAGTGATGACGCAGTCTC6890.14667969540013326No Hit
GGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC6860.14604103199490773No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG6850.14582814419316587No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACCACTGGAAGAGTAGTGATGACGCAGTCTCCAGCCACC6800.14476370518445664No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG6740.14348637837400555No Hit
GCCCTAGTGGATGCACCATAAATGAGGAGCCTGGGAGCCTGGTCAGGCTT6710.14284771496878No Hit
GTATCAACGCAGAGTACGGGGAGGAACTGCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTT6540.1392286223391686No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC6480.1379512955287175No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA6450.13731263212349196No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT6450.13731263212349196No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC6440.1370997443217501No Hit
GAGTAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAA6380.13582241751129903No Hit
GAACTGCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC6360.13539664190781533No Hit
GTCAGAGCGTCACCACCAACTTAGCCTGGTTCCAGCAGAAGCCTGACCAGGCTCCCAGGCTCCTCATTTATGGTG6340.13497086630433164No Hit
GTCGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCACTGAAGCTGG6290.13390642729562238No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA6280.13369353949388055No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA6230.1326291004851713No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAGCGTCACCACCAACTTAGCCTGGTTCCAGCAGAAGCCTGACCAGG6170.1313517736747202No Hit
GCAGAAGCCTGACCAGGCTCCCAGGCTCCTCATTTATGGTGCATCCACTAGGGCCACTGGTATCCCAGCCAGCTT6090.12964867126078544No Hit
ATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACCACTGGAAGAGTAGTGATGACG6010.12794556884685065No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGGCATAATCCCTGAATCTGAATCCAAAGCCAACACA5980.1273069054416251No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC5970.12709401763988326No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA5960.1268811298381414No Hit
GAAGAGTAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGG5880.1251780274242066No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT5830.12411358841549738No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG5800.12347492501027182No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT5760.12262337380330443No Hit
GTATCAACGCAGAGTACGGGAGGAACTGCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCT5760.12262337380330443No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT5720.12177182259633704No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC5720.12177182259633704No Hit
CACCATCGACAGCCTGCAGTCTGAGGATTTTGCAGTTTATTACTGTCAACAGTATAATAACTGGCCTCTCACTTT5710.1215589347945952No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG5580.11879139337195117No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT5570.11857850557020932No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT5460.116236739751049No Hit
CCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGCGTCACCACCAACTTAGC5440.11581096414756531No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT5380.11453363733711422No Hit
CCAGTGGCCCTAGTGGATGCACCATAAATGAGGAGCCTGGGAGCCTGGTC5370.11432074953537238No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC5330.11346919832840499No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA5330.11346919832840499No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC5320.11325631052666314No Hit
GGCTTCTGCTGGAACCAGGCTAAGTTGGTGGTGACGCTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCC5310.11304342272492128No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC5300.11283053492317943No Hit
CTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACACAGACAGGGTGGCTGG5270.1121918715179539No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC5250.11176609591447019No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA5180.11027588130227726No Hit
GGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGCGTCACCACCAACTTAGCCTGGTTCCAGCAGAAGC5170.1100629935005354No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC5120.10899855449182617No Hit
GGGCTGGAGTGGGTGGCAGTCATATGGTATGATGAAAGTCAGAAATTTTA5080.10814700328485877No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCCCGCCGCCGAAAGTG5060.10772122768137508No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT5050.10750833987963325No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA4990.10623101306918215No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG4960.10559234966395661No Hit
GGCCACTGGTATCCCAGCCAGCTTCAGTGGCAGTGGGTCTGGGACAGAGT4950.10537946186221475No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG4930.10495368625873105No Hit
GCACTAGAAGTCGGCGGTGTTTCCATTCGGTGATCAGCACTGAACACAGA4850.10325058384479627No Hit
CCTCATTTATGGTGCATCCACTAGGGCCACTGGTATCCCAGCCAGCTTCAGTGGCAGTGGGTCTGGGACAGAGTT4840.10303769604305443No Hit
GCCTGGGAGCCTGGTCAGGCTTCTGCTGGAACCAGGCTAAGTTGGTGGTG4790.10197325703434519No Hit
CTCTTCCTCCTGCTACTCTGGCTCCCAGAAACCACTGGAAGAGTAGTGATGACGCAGTCTCCAGCCACCCTGT4780.10176036923260334No Hit
GGGCCAGTCAGAGCGTCACCACCAACTTAGCCTGGTTCCAGCAGAAGCCT4780.10176036923260334No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGAGGAGCCCAGCACTAGAAG4720.10048304242215225No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT4710.1002701546204104No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT4700.10005726681866856No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACCA208.674504E-568.7325
GTGGTAT750.054.991461
GGTATCA3650.048.023361
GTATCAA10750.044.4407731
TATCAAC12450.038.64452
ATCAACG12800.037.5878143
TCAACGC12800.037.5878144
CAACGCA13050.036.867745
AACGCAG13300.036.174746
TGTTAGA4400.035.55926570
GACAGGT905.3714757E-934.369661
CGCAGAG14150.034.2372788
AGAGTAC14500.033.4108611
GCAGAGT14550.033.2960479
CGATTCA2800.032.5959970
ACGCAGA15100.031.8557367
CTTGTTC652.3586783E-531.76643432
ATAAGAG652.381122E-531.7224663
TGATATG450.004051742631.50007265
GGTAGCA450.00470381830.55406619
Produced by FastQC (version 0.11.5)