FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755852

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755852
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences366746
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7320.1995931789303769No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCATA200.006663783553.0576766
TAAACCG451.0078785E-438.2837945
GTATCAA9050.035.8661881
GGTATCA4550.033.392371
GTGGTAT1900.030.8959311
TAACGGC703.7780683E-529.64639736
TTAACGG703.792211E-529.63017535
TGGTATC1900.029.0428072
TATCAAC11400.028.7402762
CTGTCGC605.4562255E-428.7128439
TTTAACG756.0979415E-527.63592734
AACGCAG12000.027.5643316
ATCAACG11900.027.5139263
CAACGCA12000.027.2772035
TCAACGC12150.026.9404474
CAGAGTA12900.025.90833510
CGCAGAG12850.025.741018
ACGCAGA13000.025.4439967
AGAGTAC13150.025.4157811
AGTACGG10750.024.35917713
Produced by FastQC (version 0.11.5)