FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755853

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755853
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320643
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAC150.00238835668.7482810
GGTATCA4500.041.248971
GTATCAA11100.040.2580031
TATCAAC12600.035.4653852
TCAACGC12850.034.7753944
ATCAACG12950.034.506863
ACGGGGC905.336915E-934.3848716
ACGGGTA400.002636416834.38486516
CAACGCA13250.033.7255745
AACGCAG13450.033.224086
CGCAGAG14050.031.8052568
ACGAGAC553.3192322E-431.25409722
TCTGTCG553.3222703E-431.249228
ACGCAGA14350.031.1403377
AGAGTAC14450.030.68695311
AATACGG450.004701066330.5547946
GTCTTAC802.8330287E-630.0773741
GCAGAGT15050.029.691959
CAGAGTA15300.029.2067910
GGTGCGA605.530609E-428.6451178
Produced by FastQC (version 0.11.5)