FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755858

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755858
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences581736
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20790.3573786047279178No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16550.040.2516141
GGTATCA7650.035.0116651
TCAACGC19000.034.6073534
ATCAACG19200.034.2645383
CGGGATA400.00269071434.2468617
CAACGCA19250.034.157915
AACGCAG19600.033.5479476
TATCAAC19800.033.4308932
ACGCAGA21600.030.6002067
TGGTATC3700.030.5891972
CGCAGAG21750.030.2317128
AGTACGG15800.029.47831313
AGAGTAC22450.029.44162411
GTGGTAT4100.029.3132361
GAGTACG16300.028.78417212
GCAGAGT23550.028.0664319
CAGAGTA23750.027.97427710
GTACGGG17250.027.00042514
TACGGGA5800.026.57084315
TACGGGT2700.024.09964415
Produced by FastQC (version 0.11.5)