FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755862

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755862
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences701886
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29350.4181590742656215No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35100.038.6597371
GGTATCA15800.036.3188251
TATCAAC40300.033.625742
TCAACGC41600.032.6164134
ATCAACG41850.032.444693
AACGCAG42000.032.2988786
CAACGCA42250.032.0280885
TGGTATC5800.031.3846052
ACGCAGA44050.030.7957527
GTGGTAT6100.030.4349671
AGAGTAC45300.029.94597611
CAGAGTA46450.029.34951810
CGCAGAG46250.029.330878
GCAGAGT46950.029.0369559
AGTACGG36150.028.30720113
GAGTACG36650.028.01286512
TACGGGG13200.027.54307415
ACGGGTC2350.027.21944416
GTACGGG37800.026.89346714
ACGGGTA2300.026.34742716
Produced by FastQC (version 0.11.5)