FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences795713
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20500.2576305778591025No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG13550.170287528292236No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13380.16815107959779468No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13280.1668943450716527No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12920.16237010077754166No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12860.16161606006185647No Hit
CTATTAAGATGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCCGCTGATTGTGAGAGTGAATTCTGTC12730.1599823051778719No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTT12580.15809720338865899No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG12470.15671479540990282No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12310.15470402016807566No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC12280.1543269998102331No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12170.15294459183147693No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG11840.14879736789520845No Hit
ATGCTACATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCA11460.144021776695869No Hit
TCTTAATAGTTACCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT11400.1432677359801838No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTATCAGTTATTTAGCCTGGTATCAGCAAAAA11330.14238802181188442No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11140.14000022621221472No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT10960.13773810406515916No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT10830.13610434918117462No Hit
GGCCAAAAGTGTACGGAGGGTAACTATTAAGATGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCCG10670.13409357393934748No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA10650.13384222703411908No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC10640.1337165535815049No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT10590.1330881863184339No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10490.13183145179229194No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC9600.12064651450962847No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9340.11737900474165937No Hit
GGACTGAACAGAGAGAATTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT9280.11662496402597419No Hit
GGGTAACTATTAAGATGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTG9150.11499120914198963No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9000.11310610735277668No Hit
GATCTATGCTACATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA8980.1128547604475483No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA8890.11172369937402053No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC8710.109461577226965No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8570.10770214889036624No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG8520.10707378162729526No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC8480.1065710878168385No Hit
GTTGTAATGGTGCCAGCTCCTATCTTTGACCAGTGGGGCCAGGGAACCCT8210.10317790459625517No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTC8040.10104145590181385No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8020.10079010899658544No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT8000.10053876209135705No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7980.10028741518612867No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)