FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1492358
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC77240.5175701808815312No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG60050.40238334233474815No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT58940.39494544874621235No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAATACCAG56110.3759821704979636No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC55840.37417295313859006No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAATACCAGAAAAGTGTTG53060.35554471514207714No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG49000.3283394467011267No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT42220.2829079885657463No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC41750.2797586101994294No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG37290.24987301974459208No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG37250.24960498754320343No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTC37150.24893490703973173No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC36530.2447804079182073No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT36070.24169803760223751No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG34390.2304406851439132No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA33970.2276263470293321No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG32320.21657001872204926No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT31410.2104722861404569No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT31260.20946716538524937No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA31000.207724956076223No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC30720.20584873066650228No Hit
GCCTTGGGCTGACCTTGGTCCCTCCGCCGAGCACCCAGGGGGCCACACGAGGTGCGTGGAGCCTGGCCCGGGGT29960.20075611884011746No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAAGTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGC29800.19968399003456277No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC29470.19747272437310617No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA28520.19110695959012516No Hit
GTGGTGACCCAGGAGCCACCGTTCTCAGTGTCCCCTGGAGGGACAGTCACACTCACTTGTGGCTTGAGCTCTGG28440.19057089518734782No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC27230.1824629210953404No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG27200.18226189694429887No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG26600.1782414139234688No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG26380.17676723681583106No Hit
CTCCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT25770.17267974574465378No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAAGTATGTCTCCTGGTACCAACAGCA25760.1726127376943066No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC25640.17180864109014057No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC25610.17160761693909907No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC25020.1676541419686161No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTATTGCACCTCATAT24740.1657779165588954No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG24560.16457177165264636No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA24230.16236050599118979No Hit
CCTCATATGCGGGCACCAACACTTTTCTGGTATTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCCCAAGG23780.15934514372556718No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTCCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT23110.15485560435230689No Hit
CACTTAGCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCT23060.15452056410057102No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22510.15083512133147675No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC22170.14855684761967303No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA21430.14359825189398254No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC21170.14185604258495615No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG20920.14018084132627692No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT20910.14011383327592977No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG20530.13756752736273736No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA20520.13750051931239018No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT20480.1372324871110015No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC20450.13703146295996002No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA20340.13629437440614117No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG20310.13609335025509964No Hit
CATATGAGGTGCAATAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAG20290.1359593341544053No Hit
ACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAATACCAGAAAAGTGTTGG20290.1359593341544053No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGAC20250.13569130195301662No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA20160.13508822949989213No Hit
GTCTCTACTAGTCACTACGCCAGCTGGTACCAGCAGACCCCGGGCCAGGC20060.13441814899642043No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC19820.13280995578808838No Hit
GTCTCCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19620.131469794781145No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT19510.13073270622732613No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT19480.13053168207628466No Hit
GTTTGGTCCCTCCGCCGAATACCAGAAAAGTGTTGGTGCCCGCATATGAGGTGCAATAATAATCAGCCTCATCCT19090.12791836811274507No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC19060.12771734396170356No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG18670.12510402999816397No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG18610.12470198169608096No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGT18600.1246349736457338No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA18500.12396489314226213No Hit
CTACTAGTCACTACGCCAGCTGGTACCAGCAGACCCCGGGCCAGGCTCCACGCACCTCGTGTGGCCCCCTGGGTG18340.12289276433670741No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT18270.12242370798427724No Hit
GTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATCA18240.12222268383323572No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTC18160.12168661943045837No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCC18140.12155260332976404No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT18050.12094953087663951No Hit
GCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCA17900.11994441012143199No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG17870.11974338597039047No Hit
GGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCAC17860.1196763779200433No Hit
GGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGG17770.1190733054669188No Hit
GTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATCATTTATGAGGTCA17610.1180011766613641No Hit
CTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTC17520.1173981042082396No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCT17430.11679503175511506No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG17410.11666101565442073No Hit
GGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATCATTTATGAGGTC17200.11525384659713017No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17190.11518683854678301No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCA17050.11424872584192265No Hit
CCCCTGAGGGCCGCTTTCTGACCTCATAAATGATGAGTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGA17030.1141147097412283No Hit
GGATGAGGCTGATTATTATTGCACCTCATATGCGGGCACCAACACTTTTCTGGTATTCGGCGGAGGGACCAAA16670.11170241992873024No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC16390.10982619451900952No Hit
GCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGCC16230.1087540657134548No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG15940.10681083225338693No Hit
AGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA15910.10660980810234541No Hit
ATAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG15870.10634177590095673No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15870.10634177590095673No Hit
GGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGG15420.10332641363533415No Hit
CTCCCTGACCGTCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTATTGCACCTCATATGCGGGCACCAACA15370.1029913733835983No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT15230.10205326067873795No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGTCTCTGGGCTCCAGG15210.10191924457804362No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG15210.10191924457804362No Hit
GGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTG15190.10178522847734926No Hit
GTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGACATACTTATAA15110.10124916407457192No Hit
CTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGA15040.10078010772214174No Hit
GATTATTATTGCACCTCATATGCGGGCACCAACACTTTTCTGGTATTCGG15040.10078010772214174No Hit
CCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCA15000.10051207552075306No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATAA150.001157161982.53612569
CGCAAAT207.8198536E-570.196863
GTGGTAT3550.054.4036141
TGGTATC3600.053.6480062
CGTATGC605.456968E-946.14636641
GTATCAA31800.043.489591
GGTATCA11350.043.4518471
TCTCGTA801.6134436E-938.8797838
GACGTAG801.6425474E-938.8081126
AGACGTA801.6461854E-938.8016125
TATCAAC37550.036.8300632
ACGTAGA852.9704097E-936.53017427
ATCAACG38500.035.921273
TCAACGC38800.035.6435284
CAACGCA39400.035.1007355
TAGAGTA400.002601013334.4880075
AACGCAG40500.034.231396
ACGAGAC1056.475602E-1032.84131622
TCGTATG851.18045136E-732.5585840
CGCAGAG43250.032.0516058
Produced by FastQC (version 0.11.5)