FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755866

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755866
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1492358
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC73240.49076696074266357No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG61260.4104913164267555No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT56200.37658524295108814No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC54990.3684772688590807No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAATACCAG51030.3419420809216019No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG49740.33329804242681715No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAATACCAGAAAAGTGTTG48540.3252570763851569No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT42110.2821709000119274No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC40360.2704444912011729No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG39490.2646147908209692No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG36760.2463215930761922No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT34470.23097674954669054No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG34060.2282294194824566No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC33760.22621917797204155No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTC33310.22320381570641898No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA32530.2179771877793398No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG31700.2124155196005248No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC31490.21100835054323427No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA31460.21080732639219274No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAAGTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGC31370.21020425393906825No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT30810.20645180311962677No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC29240.1959315392151213No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT28530.19117396764047231No Hit
GTGGTGACCCAGGAGCCACCGTTCTCAGTGTCCCCTGGAGGGACAGTCACACTCACTTGTGGCTTGAGCTCTGG28250.1892977422307516No Hit
GCCTTGGGCTGACCTTGGTCCCTCCGCCGAGCACCCAGGGGGCCACACGAGGTGCGTGGAGCCTGGCCCGGGGT27910.18701946851894788No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG26820.1797155910311065No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAAGTATGTCTCCTGGTACCAACAGCA26350.17656621266478953No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC26320.17636518851374805No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG26000.17422093090263865No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA25780.17274675379500093No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC25540.17113856058666888No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG24950.16718508561618592No Hit
CTCCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT24850.16651500511271425No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTATTGCACCTCATAT24820.16631398096167274No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC23610.15820600686966532No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC23280.15599474120820875No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTCCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22700.15210827428807297No Hit
CCTCATATGCGGGCACCAACACTTTTCTGGTATTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCCCAAGG21810.14614455780717495No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC21590.14467038069953725No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC21380.1432632116422467No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA21350.1430621874912052No Hit
CACTTAGCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCT21340.14299517944085802No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG20930.1402478493766241No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG20750.1390417044703751No Hit
GTCTCTACTAGTCACTACGCCAGCTGGTACCAGCAGACCCCGGGCCAGGC20520.13750051931239018No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20420.13683043880891851No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA19880.1332120040901714No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT19860.13307798798947706No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA19490.1305986901266318No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC19410.13006262572385446No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT19320.12945955327072994No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA19260.12905750496864693No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG19250.12899049691829978No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA19050.1276503359113564No Hit
GTTTGGTCCCTCCGCCGAATACCAGAAAAGTGTTGGTGCCCGCATATGAGGTGCAATAATAATCAGCCTCATCCT19030.12751631981066205No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC19020.12744931176031488No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA18970.12711427150857904No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG18910.12671222320649603No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG18690.12523804609885833No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT18630.12483599779677532No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGAC18460.12369686094087344No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG18230.12215567578288855No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC18230.12215567578288855No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT18220.12208866773254139No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT18200.12195465163184706No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG18120.12141858722906969No Hit
GGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGG18020.12074850672559802No Hit
GGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCAC17950.12027945037316784No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCT17940.12021244232282066No Hit
ACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAATACCAGAAAAGTGTTGG17880.11981039402073765No Hit
GTCTCCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17700.11860424911448861No Hit
CCCCTGAGGGCCGCTTTCTGACCTCATAAATGATGAGTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGA17580.11780015251032258No Hit
GTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATCA17440.11686203980546223No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGT17410.11666101565442073No Hit
CTACTAGTCACTACGCCAGCTGGTACCAGCAGACCCCGGGCCAGGCTCCACGCACCTCGTGTGGCCCCCTGGGTG17400.11659400760407357No Hit
GTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATCATTTATGAGGTCA17350.11625896735233772No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTC17300.11592392710060187No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCA17270.11572290294956035No Hit
GCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCA17220.11538786269782453No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCC17200.11525384659713017No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT17020.11404770169088113No Hit
CATATGAGGTGCAATAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAG16830.11277454873428493No Hit
CTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTC16680.11176942797907741No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT16610.11130037162664723No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16430.1100942267203982No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG16380.10975918646866235No Hit
CTCCCTGACCGTCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTATTGCACCTCATATGCGGGCACCAACA16100.10788296105894161No Hit
GGATGAGGCTGATTATTATTGCACCTCATATGCGGGCACCAACACTTTTCTGGTATTCGGCGGAGGGACCAAA15990.10714587250512275No Hit
GATTATTATTGCACCTCATATGCGGGCACCAACACTTTTCTGGTATTCGG15920.10667681615269257No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC15850.1062077598002624No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15840.10614075174991523No Hit
GGTTATAAGTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATCATTTATGAGGTCAGAAAG15600.10453255854158319No Hit
GGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATCATTTATGAGGTC15550.10419751828984734No Hit
AGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA15330.10272334118220965No Hit
CTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGA15060.10091412382283607No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAATAC14930.10004301916832288No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4000.051.796511
GGGCGTA200.00741061651.65941613
CGGTCGT200.00741061651.65941610
AATCGAG554.920589E-448.38062770
TGGTATC4500.047.5249672
GTATCAA32750.043.651411
GGTATCA12100.039.667851
GCTTAGT350.001342006839.464011
TATCAAC36450.038.989022
ATCAACG38650.036.554433
TCAACGC38900.036.0332154
CAACGCA39550.035.3539355
AACGCAG40150.035.082946
CTTCGGT501.8636098E-434.4939272
TTGCGCG400.002618125434.44191718
CGCAGAG42100.033.294358
AGAGTAC42950.032.87599611
CAGAGTA43450.032.49767310
GCAGAGT44150.032.060439
ACGCAGA43900.032.0076527
Produced by FastQC (version 0.11.5)