FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755867

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755867
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1450842
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATACCGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC141730.9768810111645513No Hit
GTATAATACTTATTGGGGATCTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT121800.8395125037736708No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT87060.6000653413672887No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC66950.46145617510383624No Hit
GAGTATTGGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTCTGATGCCTC61960.42706235413642557No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC58500.4032141335858763No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC56000.38598276035571066No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC54250.37392079909459475No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG52820.36406445360693995No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT52730.363444124170654No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG49740.34283540178737587No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG49730.3427664762944552No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA43210.2978270549101832No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTGGTAGCTGGTTGGCCTGGTATCAGCAGAAA43160.2974824274455799No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA41750.28776393294376645No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT40620.27997535224373155No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG40140.27666692858353975No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGAT40060.27611552464017447No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC39840.2745991637959199No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG39620.2730828029516653No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT39450.27191106957201405No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT38930.26832694394013956No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG36710.2530254845117525No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA36660.2526808570471492No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT36390.25081986873829126No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC35590.2453058293046383No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC35560.24509905282587627No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT35130.2421352566302878No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT34490.23772402508336538No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG33810.23303709156476032No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG33510.23096932677714044No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31690.21842488706557986No Hit
GAGTGAATTCTGTCCCAGATCCAGTGCCGCTGAACCTTGATGGGACCCCA31260.21546109086999135No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC30710.2116701887593549No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA30210.2082239141133218No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA29320.20208954524338282No Hit
GTATTGGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT28680.19767831369646038No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTCTGATGCCTCCAGTTTGGAAAGTGGGGTCCCAT28050.19333600764245865No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT27610.1903032859539495No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG27350.18851122313801227No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT27060.18651238384331306No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTGGTAG27030.18630560736455107No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG26660.18375536412648655No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC26590.1832728856760419No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA26380.18182545032470798No Hit
GACTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG26270.1810672699025807No Hit
GGCCAAAAGATCCCCAATAAGTATTATACCGTTGGCAGTAATAAGTTGCA25510.17582893244061035No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT24600.16955671258483004No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAAC23820.16418052413701836No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA23610.16273308878568443No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG23540.16225061033523983No Hit
GCACTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCT23290.16052747301222325No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC23110.15928681413965132No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC22920.15797722977415873No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCAGTGCCG22910.15790830428123806No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA22870.15763260230955542No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA22780.15701227287326947No Hit
ACCTTGATGGGACCCCACTTTCCAAACTGGAGGCATCAGAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTG22600.15577161400069753No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA22180.1528767432980297No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC22010.15170500991837843No Hit
CATCAAGGTTCAGCGGCACTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTG21820.15039542555288582No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC21690.14949939414491722No Hit
GTATCAACGCAGAGTACGGGAGTCAGACTCAGTCAGGACACAGCATGGAC21620.1490169156944726No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC21360.14722485287853537No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT21330.14701807639977338No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG21120.14557064104843945No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA20960.14446783316170886No Hit
GTCAGAGTATTGGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTCTGATG20730.1428825468245336No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT20560.14171081344488234No Hit
ATCCAGTGCCGCTGAACCTTGATGGGACCCCACTTTCCAAACTGGAGGCA20230.1394362721785005No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT20160.13895379372805583No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC20070.13833346429176988No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG20040.1381266878130079No Hit
GTCCCAGATCCAGTGCCGCTGAACCTTGATGGGACCCCACTTTCCAAACT20030.13805776232008724No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT19940.13743743288380125No Hit
CCCCTAAGCTCCTGATCTCTGATGCCTCCAGTTTGGAAAGTGGGGTCCCA19600.13509396612449873No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC19430.13392223274484746No Hit
GCTGATGGTGAGAGTGAATTCTGTCCCAGATCCAGTGCCGCTGAACCTTG19160.13206124443598957No Hit
GCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACCCTGTCTGCATCTGTAGGAGA19130.13185446795722758No Hit
GGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCAGTGCC18800.12957992669084573No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18550.12785678936782918No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA18520.1276500128890672No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT18380.12668505598817792No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC18330.1263404285235746No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC18200.12544439711560598No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC18180.12530654612976463No Hit
GGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGA17940.12365233429966875No Hit
GTAATAAACAGCCGTGTCTGCGGCGGTCACAGAGGTCTGCTTCAGGGAGA17930.12358340880674808No Hit
GCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTCTGATGCCTCCAGTTTGGAAAGTGGGGTCCCATCAAGGTT17850.12303200486338278No Hit
ACTTATTACTGCCAACGGTATAATACTTATTGGGGATCTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA17720.12213597345541417No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC17500.12061961261115958No Hit
GCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACCA17330.11944787923150832No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC17190.11848292233061905No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT17090.11779366740141241No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG16930.11669085951468182No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC16810.11586375359963386No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16810.11586375359963386No Hit
GAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTGGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAG16750.11545020064210988No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA16740.11538127514918924No Hit
CCAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA16680.11496772219166526No Hit
CTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTGGT16660.11482987120582393No Hit
CTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTC16620.11455416923414129No Hit
CTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGG16460.11345136134741068No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA16430.11324458486864869No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC16420.11317565937572803No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT16290.11227962796775942No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC16090.11090111810934616No Hit
CTCCTGATCTCTGATGCCTCCAGTTTGGAAAGTGGGGTCCCATCAAGGTT15940.10986723571553621No Hit
ACCCAGTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCAT15890.10952260825093292No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT15820.10904012980048827No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC15590.10745484346331304No Hit
CACCATCACTTGCCGGGCCAGTCAGAGTATTGGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCC15460.10655881205534441No Hit
GTCTCTGGTGGCTCCATCAGCAGTAATGATTACTACTGGGGCTGGATCCG15340.10573170614029646No Hit
GTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCA15270.10524922768985182No Hit
CCTGATCTCTGATGCCTCCAGTTTGGAAAGTGGGGTCCCATCAAGGTTCAGCGGCACTGGATCTGGGACAGAATT15050.10373286684559725No Hit
GGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC15050.10373286684559725No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG14870.10249220797302531No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG14780.10187187853673936No Hit
GGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC14770.1018029530438187No Hit
GCCCCTAAGCTCCTGATCTCTGATGCCTCCAGTTTGGAAAGTGGGGTCCCATCAAGGTTCAGCGGCACTGGAT14730.10152725107213603No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA14670.10111369811461207No Hit
ATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATT14570.10042444318540543No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG1050.052.7152240
TGCCGTC1100.050.43971645
ACGAGAC1150.047.87573622
GTGGTAT2400.047.3016471
CGTATGC1200.046.1498241
ATGCCGT1250.044.3684544
GTATCAA27550.042.3303261
CTCGTAT1350.040.98510739
GCCGTCT1450.038.2885646
TACGGAT5850.037.54542570
TATGCCG1500.036.94932643
TATCAAC31800.036.671712
TCCGAGC1600.036.5487513
CGACCGG700.00214747635.8597170
ATCTCCG1550.035.5072410
ATCAACG33100.035.231433
TCAACGC33350.034.9673274
CAACGCA33600.034.7071535
AACGCAG34000.034.2988366
CACGAGA1750.033.42521721
Produced by FastQC (version 0.11.5)