FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755868

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755868
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1450842
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATACCGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC143990.9924581725646211No Hit
GTATAATACTTATTGGGGATCTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT131910.9091961771164607No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT86030.5929660155964606No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC58480.403076282600035No Hit
GAGTATTGGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTCTGATGCCTC58310.4019045492203837No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC55730.38412177204685277No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC55530.38274326218843957No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT53100.3659943674087185No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC51450.3546216610768092No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG50050.3449720920679164No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG43330.29865416082523116No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA41390.2852826151986226No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC39980.27556412069680913No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA39710.27370313238795124No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT39000.26880942239058425No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG38520.2655009987303924No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGAT38460.26508744577286847No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTGGTAGCTGGTTGGCCTGGTATCAGCAGAAA38450.26501852027994777No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT38250.2636400104215345No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT37610.25922877887461215No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT36370.25068201775244997No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG36140.2490967314152747No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG35620.24551260578340026No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT35200.24261773508073245No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG35010.24130815071523984No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA34210.23579411128158684No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC33630.23179643269218841No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC32790.22600669128685275No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG32770.2258688403010114No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT32370.2231118205841849No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA31730.21870058903726253No Hit
GAGTGAATTCTGTCCCAGATCCAGTGCCGCTGAACCTTGATGGGACCCCA29890.20601829833986057No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG29510.2033991296088754No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT28880.19905682355487367No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28230.19457666651503058No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG27750.19126824285483876No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA27700.19092361539023545No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA27630.19044113693979084No Hit
GTATTGGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT27370.1886490741238536No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC27000.18609883088578907No Hit
GGCCAAAAGATCCCCAATAAGTATTATACCGTTGGCAGTAATAAGTTGCA25930.17872380314327818No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTCTGATGCCTCCAGTTTGGAAAGTGGGGTCCCAT25580.17631141089105498No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG25450.17541537948308636No Hit
GACTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG25350.17472612455387973No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT24430.16838497920517878No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTGGTAG24410.16824712821933746No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT23710.1634223437148911No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT23500.16197490836355716No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCAGTGCCG22970.15832185723876202No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA22920.15797722977415873No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAAC22870.15763260230955542No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA22770.1569433473803488No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA22660.1561851669582215No Hit
ACTTATTACTGCCAACGGTATAATACTTATTGGGGATCTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA22150.1526699668192677No Hit
GCACTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCT21720.14970617062367922No Hit
GTATCAACGCAGAGTACGGGAGTCAGACTCAGTCAGGACACAGCATGGAC21400.147500554850218No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA21290.1467423744280907No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA21230.14632882147056675No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC21090.14536386456967745No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC21060.1451570880909155No Hit
CATCAAGGTTCAGCGGCACTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTG20940.14432998217586754No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG20900.1440542802041849No Hit
ACCTTGATGGGACCCCACTTTCCAAACTGGAGGCATCAGAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTG20600.141986515416565No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC20480.14115940950151706No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC20260.1396430486572625No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC19970.13764420936256325No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT19850.1368171034475153No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC19800.13647247598291198No Hit
ATCCAGTGCCGCTGAACCTTGATGGGACCCCACTTTCCAAACTGGAGGCA19780.13633462499707066No Hit
GTCCCAGATCCAGTGCCGCTGAACCTTGATGGGACCCCACTTTCCAAACT19630.13530074260326072No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19480.13426686020945078No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19270.13281942485811687No Hit
GGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCAGTGCC19240.13261264837935488No Hit
GTCAGAGTATTGGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTCTGATG19190.13226802091475157No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT19160.13206124443598957No Hit
CCCCTAAGCTCCTGATCTCTGATGCCTCCAGTTTGGAAAGTGGGGTCCCA19000.13095843654925898No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA18970.130751660070497No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC18620.1283392678182738No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC18560.12792571486074983No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA18500.12751216190322587No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT18460.1272364599315432No Hit
GCTGATGGTGAGAGTGAATTCTGTCCCAGATCCAGTGCCGCTGAACCTTG18420.12696075795986056No Hit
GCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACCCTGTCTGCATCTGTAGGAGA18230.12565117359436795No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG18010.12413481275011337No Hit
GGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGA17940.12365233429966875No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT17720.12213597345541417No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC17610.12137779303328687No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT17550.12096424007576291No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC17300.11924110275274634No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC17270.11903432627398434No Hit
GTAATAAACAGCCGTGTCTGCGGCGGTCACAGAGGTCTGCTTCAGGGAGA17220.11868969880938103No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC17090.11779366740141241No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC16910.1165530085288405No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG16910.1165530085288405No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT16880.1163462320500785No Hit
GCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTCTGATGCCTCCAGTTTGGAAAGTGGGGTCCCATCAAGGTT16790.11572590261379255No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCACTGGATCTGGGACAGAATTCACT16540.11400276529077598No Hit
CTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTC16480.113589212333252No Hit
CCAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA16440.11331351036156935No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG16310.11241747895360074No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA16090.11090111810934616No Hit
CACCATCACTTGCCGGGCCAGTCAGAGTATTGGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCC15960.11000508670137754No Hit
GAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTGGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAG15960.11000508670137754No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT15890.10952260825093292No Hit
ACCCAGTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCAT15730.1084198003642023No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT15650.10786839642083701No Hit
GTCTCTGGTGGCTCCATCAGCAGTAATGATTACTACTGGGGCTGGATCCG15630.10773054543499568No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC15500.10683451402702707No Hit
CTCCTGATCTCTGATGCCTCCAGTTTGGAAAGTGGGGTCCCATCAAGGTT15320.10559385515445513No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC15230.10497352571816917No Hit
GCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACGGTATAATACTTATTGGGGATCTTTTGGCCAGGGGA15180.10462889825356586No Hit
GGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC15160.10449104726772454No Hit
CTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGG15120.10421534529604189No Hit
TCTCTGATGCCTCCAGTTTGGAAAGTGGGGTCCCATCAAGGTTCAGCGGCACTGGATCTGGGACAGAATTCACT15090.10400856881727989No Hit
GCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACCA15030.10359501585975592No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCC14960.10311253740931127No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC14940.10297468642346996No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC14930.10290576093054929No Hit
CCTGATCTCTGATGCCTCCAGTTTGGAAAGTGGGGTCCCATCAAGGTTCAGCGGCACTGGATCTGGGACAGAATT14810.10207865501550134No Hit
GGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC14700.10132047459337405No Hit
CTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTGGT14650.10097584712877074No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT14550.10028659219956412No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA14550.10028659219956412No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCGG451.8922765E-645.8914530
TACGGAT6250.044.2661670
GTGGTAT3900.044.1324921
GTATCAA28000.041.3080141
TATCAAC30950.036.9885032
ATCAACG32300.035.4132163
TCAACGC32600.034.9723134
GTAGTCG601.36727485E-534.40907729
CAACGCA33300.034.1281555
CCGACAT952.0760502E-433.86334270
AACGCAG33950.033.5758746
GACAGGT2450.032.3157921
CGCAGAG35300.032.194558
GTCTAGC751.6988779E-632.1284521
AGTCGGT652.3491692E-531.79187231
AGAGTAC36300.031.49681511
ATCAGGC38250.031.20643236
GCAGAGT36950.030.7569059
TTCTGTC45650.030.58908369
GCAGGCT40300.029.53298444
Produced by FastQC (version 0.11.5)