FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755869

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755869
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences376262
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA551.5189835E-743.712133
GGTATCA5550.042.086261
GTATCAA13700.041.6209451
GTGGTAT2050.040.2144171
TGGTATC2200.037.467542
TATCAAC16100.035.4118732
GATCGTT400.002651945234.3452512
ATCAACG17300.032.9555553
TCAACGC17450.032.672274
CAACGCA17800.032.029845
AACGCAG18100.031.4989576
TGGTCGG450.00408877231.43992862
ACGCAGA18900.030.1656687
AGAGTAC19050.029.92814411
CGCAGAG19150.029.7718628
CAGAGTA19500.029.23749410
GTATTAT605.5553747E-428.6248441
GTATTAG605.5553747E-428.6248441
AGTACTA500.0078998227.4761985
GCAGAGT20900.027.2790019
Produced by FastQC (version 0.11.5)