FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755871

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755871
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1243091
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47450.38170978633100877No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39010.3138145155905722No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33380.2685241868857549No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30470.24511479851434848No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30240.24326457194203802No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29790.2396445634309958No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28090.22596897572261404No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27660.22250985647872926No Hit
GTAATATGTTGCAAGATCTTCAGGTTGCAGGCTGCTGATGGTGAGGGTAAAATTTGCCCCAGATCCACTTCCG27180.21864851406695082No Hit
ACATATTACTGTCAACAGTTTCATAATTTACCGATCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAGCGAA25620.20609915122867112No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25610.2060187065950924No Hit
GTGCAGCCACAGTTCGCTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG25000.2011115839467907No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24640.19821557713795693No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24350.19588268276417414No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24100.19387156692470625No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23810.19153867255092347No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG23540.18936666744429814No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGG23520.18920577817714068No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22580.18164398262074136No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22500.18100042555211163No Hit
ATATTACTGTCAACAGTTTCATAATTTACCGATCACTTTCGGCGGAGGGA22050.17738041704106938No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21040.16925550904961906No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG20690.16643994687436398No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20170.16225682592827073No Hit
GTGGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGAAATCATCAACTATTTAAATTGGTATCAGCAGCAA19850.1596825976537518No Hit
GGGTAAAATTTGCCCCAGATCCACTTCCGCTGAACCTTGACGGGACCCCT19220.15461458573829268No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18870.15179902356303762No Hit
CACCATCAGCAGCCTGCAACCTGAAGATCTTGCAACATATTACTGTCAACAGTTTCATAATTTACCGATCACTTT18190.14632878847968492No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17840.14351322630442984No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17820.1433523370372724No Hit
GTCTCCTTCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGAAATCATCAA17700.1423870014343278No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC17570.1413412211978045No Hit
CCTTCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCA17340.13949099462549402No Hit
GTAATACACGCCCGTGTCCTCGACTCTCAGGCTGCTCATTTGGAGATACA17120.13772121268676227No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17000.13675587708381767No Hit
CAGTAATATGTTGCAAGATCTTCAGGTTGCAGGCTGCTGATGGTGAGGGTAAAATTTGCCCCAGATCCACTTCCG16970.13651454318308154No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGGTGTGCCAT16580.1333772024735116No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATCTCCACCTTGGTCC15900.1279069673901589No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15850.12750474422226532No Hit
ATTTTACCCTCACCATCAGCAGCCTGCAACCTGAAGATCTTGCAACATATTACTGTCAACAGTTTCATAATTTAC15700.12629807471858456No Hit
CTCCTGATCTGCGATGCATCGAAATTGGAACCAGGGGTCCCGTCAAGGTT15620.12565451764995483No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15550.12509140521490383No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT15480.1245282927798528No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15360.1235629571769082No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC15050.12106917353596801No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC14740.1185753898950278No Hit
GCTGATGGTGAGGGTAAAATTTGCCCCAGATCCACTTCCGCTGAACCTTG14650.11785138819281935No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT14590.11736872039134706No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG14440.11616205088766632No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG14440.11616205088766632No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14400.11584027235335144No Hit
ATCTTCAGGTTGCAGGCTGCTGATGGTGAGGGTAAAATTTGCCCCAGATCCACTTCCGCTGAACCTTGACGGGAC14340.11535760455187916No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14280.11487493675040686No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14200.11423137968177711No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTGCTGCTGATACCAATTTAAAT13720.11037003726999874No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13640.10972648020136899No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13520.10876114459842441No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13380.1076349197283224No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13340.1073131411940075No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13310.10707180729327137No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13290.10691091802611392No Hit
CCTGAAGATCTTGCAACATATTACTGTCAACAGTTTCATAATTTACCGAT13250.10658913949179907No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT13180.10602602705674807No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13000.10457802365233117No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGGTGTGCCATCCAGATGACCCAGTCTCCTTCCTCC12890.10369313268296527No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12840.1032909095150717No Hit
ATCCAGATGACCCAGTCTCCTTCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCAGGCGAGT12750.10256690781286326No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12640.10168201684349737No Hit
GACCAAGGTGGAGATCAAGCGAACTGTGGCTGCACCATCTGTCTTCATCT12590.10127979367560379No Hit
GCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGGTGTGCCATCCAGATGACCCAGTCTCCTTC12460.10023401343908049No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25850.041.499821
TATCAAC27600.038.6146742
ATCAACG29300.036.139563
TCAACGC29900.035.5293354
CAACGCA30100.035.2932555
AATCGCA400.002597857734.49608634
AACGCAG31050.034.2134326
GTGGTAT3400.033.372381
CGCAGAG32900.032.285688
AGAGTAC33100.031.88289311
GCAGAGT33900.031.3332989
CAGAGTA34200.031.2594710
CGTATGC1001.3951649E-831.17356541
ACGCAGA35200.030.2737627
GCCGTCT1352.8012437E-1028.32285146
AGTACGG26650.027.7335913
GAGTACG27150.027.22174512
CGAGACG1404.838512E-1027.02552223
AGACGTA1306.493792E-926.46601125
ATCTCCG1306.555638E-926.44258910
Produced by FastQC (version 0.11.5)