FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755873

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755873
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12168
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT8827.2485207100591715No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT2181.7915844838921762RNA PCR Primer, Index 17 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT1701.3971071663379357RNA PCR Primer, Index 17 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATGTCGTATGCCGT410.33694937541091385No Hit
TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCT370.30407626561472717No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC340.2794214332675871No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT330.27120315581854043No Hit
GTATTGCGCGGCTCGAATAGAATGCAAATTGTCGCCGAGTTACGGAACAA300.2465483234714004No Hit
TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC290.23833004602235372No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250.20545693622616698No Hit
TGTCAAAGGTGGGTAAGGTTTTTCGCGTTGCATCGAATTAAACCACATCA230.18902038132807364No Hit
GTGTTGGTCAGCGTCTCGGCTGCCACGCGGCTGCGGGGGTCCTGCTCGAA220.18080210387902695No Hit
GTATCAACGCAGAGTACGGGTGCCTGCGCAGAGAAATGG220.18080210387902695No Hit
CCATTTCTCTGCGCAGGCACCCGTACTCTGCGTTGATAC210.17258382642998027No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210.17258382642998027No Hit
GTACAAAGGGTTGCCAACCCGCGAGGGGGAGCTAATCCCATAAAACCAGTCGTAGTCCGGATCGCAGTCTGCAA200.1643655489809336No Hit
CCGCAGAGTTGGCAACGCGCCAAGTCAAATCAAGGAGTTTTGAACAAATG190.15614727153188693No Hit
CCTTTGACATGTACGGAATTTGCCAGAGATGGCTTAGTGCTCGAAAGAGAACCGTAACACAGGTGCTGCATGG190.15614727153188693No Hit
GTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAA180.14792899408284024No Hit
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTG180.14792899408284024TruSeq Adapter, Index 12 (95% over 23bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.14792899408284024No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATGTCGTATGCCGTCTT170.13971071663379356No Hit
GACCAACACCGGCCTCGTGGTGCTGGCGGGCGAAATCACCACCAACGCGCACGTTGACTACATCCAGGTCGCGCG160.13149243918474687No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.13149243918474687No Hit
CTGCTAAAGCGTTCGCCAGAGCTCCGCAGAGTTGGCAACGCGCCAAGTCA160.13149243918474687No Hit
TCCGAGATCTGGTCGGCGACCTTGTCGGGATGGCCTTCGGAGACGGATTC160.13149243918474687No Hit
CCCTAAACGATGTCAACTGGTTGTTGGGTCTTCACTGACTCAGTAACGAA160.13149243918474687No Hit
GTATACATGCTTCGTGGAGGGGTGTGACAGGAAAAGCGCCACACCGGACA150.1232741617357002No Hit
GTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACAGGCAGA150.1232741617357002No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.1232741617357002No Hit
GTACAAGACCCGGGAACGTATTCACCGTGACATTCTGATCCACGATTACT150.1232741617357002No Hit
GTGTAGCCCCACCTATAAGGGCCATGAGGACTTGACGTCATCCCCACCTT150.1232741617357002No Hit
CTCTAATGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCA150.1232741617357002No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.1232741617357002No Hit
GGCGGAAGTCGTCCGGCCGGCACGCAAGTATAAAGGACGAACAGAGAAGGGCAAGCATTGCGGAGTCGTCATCGT150.1232741617357002No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.11505588428665352No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.11505588428665352No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT140.11505588428665352No Hit
GTGTGTACAAGACCCGGGAACGTATTCACCGTGACATTCTGATCCACGAT130.10683760683760685No Hit
GTGTTACGGTTCTCTTTCGAGCACTAAGCCATCTCTGGCAAATTCCGTAC130.10683760683760685No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.10683760683760685No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG1200.074.1156170
GGCCGGC200.004244342459.0207259
CTTATAC950.055.922891
TTATACA1000.053.1267472
GGGGGGG1450.051.9005970
TGAAAAA1300.051.45396459
TTGAAAA1300.050.9262358
CTTGAAA1300.050.9262357
TGCTTGA1250.049.84779455
GCTTGAA1250.049.84779456
AAAGGGG1100.049.5841368
CTGCTTG1250.049.34172454
CTCGTAT1250.049.1801939
TTCTGCT1250.049.0925252
TCTGCTT1250.049.0925253
AAGGGGG1200.048.67948569
TATACAC1100.048.2970473
ATCTCGT1250.048.26093337
TCTCGTA1250.048.26093338
GTCTTCT1350.048.05434449
Produced by FastQC (version 0.11.5)