FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755874

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755874
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12168
Sequences flagged as poor quality0
Sequence length20-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG179914.784681130834976No Hit
GTGTTGGTCAGCGTCTCGGCTGCCACGCGGCTGCGGGGGTCCTGCTCGAA260.2136752136752137No Hit
GTATTGCGCGGCTCGAATAGAATGCAAATTGTCGCCGAGTTACGGAACAA250.20545693622616698No Hit
CCATTTCTCTGCGCAGGCACCCGTACTCTGCGTTGATAC230.18902038132807364No Hit
GTATCAACGCAGAGTACGGGTGCCTGCGCAGAGAAATGGCGGGACAAAAA230.18902038132807364No Hit
CTGCTAAAGCGTTCGCCAGAGCTCCGCAGAGTTGGCAACGCGCCAAGTCA220.18080210387902695No Hit
GTATCAACGCAGAGTACGGGTGCCTGCGCAGAGAAATGG180.14792899408284024No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT180.14792899408284024No Hit
ACCTGGATGTAGTCAACGTGCGCGTTGGTGGTGATTTCGCCCGCCAGCACCACGAGGCCGGTGTTGGTCAGCGT170.13971071663379356No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT160.13149243918474687No Hit
CCTTTGACATGTACGGAATTTGCCAGAGATGGCTTAGTGCTCGAAAGAGAACCGTAACACAGGTGCTGCATGG160.13149243918474687No Hit
GGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCAACTGGTTGTT150.1232741617357002No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT140.11505588428665352No Hit
GGGCTACACACGTCATACAATGGCTGGTACAAAGGGTTGCCAACCCGCGA140.11505588428665352No Hit
GTATCAACGCAGAGTACGGGTGCCTGCGCAGAGAAATGGCGGGATAAAAA140.11505588428665352No Hit
GCTGCGGGGGTCCTGCTCGAAGATCGCGTCGAGGATGGCGTCCGAGATCTGGTCGGCGACCTTGTCGGGATGGC140.11505588428665352No Hit
GTATCAACGCAGAGTACGGGAGGGAGTCAAAACACGGACGCGCGCGCATG140.11505588428665352No Hit
GCTCCAGTCAGATCATCCATATCAACATCCGGGACTTCGCGCGGATCCTT140.11505588428665352No Hit
GCATTGCGGAGTCGTCATCGTCTGCAGAAGGAAAACCAGAGGCGGCACCA130.10683760683760685No Hit
GTGTGTACAAGACCCGGGAACGTATTCACCGTGACATTCTGATCCACGAT130.10683760683760685No Hit
GTACAAAGGGTTGCCAACCCGCGAGGGGGAGCTAATCCCATAAAACCAGTCGTAGTCCGGATCGCAGTCTGCAA130.10683760683760685No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.10683760683760685No Hit
TGCCGGGACCTTGCCAATGTGGACGATCGAACTACATTAGTTGCGCTTTC130.10683760683760685No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT130.10683760683760685No Hit
GCTCGAAGATCGCGTCGAGGATGGCGTCCGAGATCTGGTCGGCGACCTTGTCGGGATGGCCTTCGGAGACGGATT130.10683760683760685No Hit
GTGCGATTGTGCCGGGCCGAGAAATCGGCATCTGCTAAAGCGTTCGCCAG130.10683760683760685No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1350.030.640451
GGTATCA706.164581E-527.2733691
ACGGGTG608.015447E-426.62534516
TATCAAC1659.094947E-1225.069462
AGTACGG1551.07320375E-1024.6340113
GAGTACG1551.07320375E-1024.6340112
TACGGGT650.001278895424.5772415
AACGCAG1751.8189894E-1123.6369196
GTACGGG1652.3646862E-1023.1410414
ACGCAGA1802.7284841E-1122.9803377
AGAGTAC1953.6379788E-1222.8443611
ATCAACG1853.8198777E-1122.359253
CAGAGTA1905.638867E-1121.77084710
GCAGAGT1905.638867E-1121.7708479
TCAACGC1807.021299E-1021.212624
CGCAGAG1957.8216544E-1121.212628
CAACGCA1859.877112E-1020.6393075
Produced by FastQC (version 0.11.5)