FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755875

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755875
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences837693
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30200.3605139352961049No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG21550.2572541491930815No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21380.2552247661136001No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19310.23051404273403264No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG18960.22633590109980625No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18790.2243065180203249No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18680.22299338779242514No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18020.21511460642502683No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC18020.21511460642502683No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17740.21177209311764572No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTT17710.21141396669185486No Hit
TCTTAATAGTTACCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT16560.19768578703653963No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT16530.1973276606107488No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC16280.19434327372915852No Hit
CTATTAAGATGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCCGCTGATTGTGAGAGTGAATTCTGTC15870.1894488792433505No Hit
GGACTGAACAGAGAGAATTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT15850.18921012829282327No Hit
ATGCTACATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCA15610.1863451168864966No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTATCAGTTATTTAGCCTGGTATCAGCAAAAA15560.18574823951017855No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15410.1839576073812244No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA15360.18336073000490635No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15320.18288322810385188No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT15210.18157009787595219No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG14910.17798883361804385No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14770.17631757696435327No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT14640.17476569578592635No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC14240.1699906767753819No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13870.16557378419062832No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13800.16473815586378304No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13430.16032126327902943No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12870.15363623666426723No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTC12820.15303935928794918No Hit
GGCCAAAAGTGTACGGAGGGTAACTATTAAGATGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCCG12800.15280060833742193No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC12590.1502937233568861No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12390.1479062138516139No Hit
GTTGTAATGGTGCCAGCTCCTATCTTTGACCAGTGGGGCCAGGGAACCCT12120.14468307601949643No Hit
GGGTAACTATTAAGATGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTG11970.14289244389054226No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11800.14086306081106084No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC11650.1390724286821067No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11590.13835617583052504No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11550.1378786739294706No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT11340.13537178894893476No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG11330.13525241347367115No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC11140.13298427944366253No Hit
GATCTATGCTACATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA10710.12785113400732726No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10580.1262992528289003No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10550.1259411264031095No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10490.12522487355152784No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGAT10420.12438924522468255No Hit
GGGCATTATCAGTTATTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTACATC10310.12307611499678282No Hit
CTCCTGTGCAGCCTCTGGATTCGCCTTTAGAGGCTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG10200.1217629847688831No Hit
TAGTTACCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT10200.1217629847688831No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG9920.118420471461502No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9750.11639108838202063No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC9730.11615233743149339No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9680.11555546005517535No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG9670.11543608457991172No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT9460.11292919959937589No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9350.11161606937147618No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9310.11113856747042175No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9300.11101919199515813No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9280.1107804410446309No Hit
GATCAGGACTGAACAGAGAGAATTCACCATGGAGTTTGGGCTGAGCTGGC9280.1107804410446309No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9240.11030293914357646No Hit
ATAATATACGGCCGTGTCCTCGGCTCTCAGGCTGCTCATTTGCAGAAACA9130.10898980891567674No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9080.10839293153935868No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9070.10827355606409508No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA8840.10552792013303204No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCTAAAT8810.1051697937072412No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA8600.10266290872670536No Hit
GTATCAACGCAGAGTACGGGAGTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT8560.10218540682565092No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGA8440.1007529011224876No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGTA200.00722886751.9828542
ACGGGTA306.308531E-446.002873
GTATCAA17250.045.8028531
TATCAAC19150.041.4386142
ATCAACG20500.038.878033
TCAACGC20750.038.2433474
CAACGCA21200.037.4315765
AACGCAG21500.037.069756
AGAGTAC23400.034.2052211
CAGAGTA23500.033.91285310
CGCAGAG23700.033.626678
GCAGAGT24150.033.0000849
GGCGCGT751.6491394E-632.24820732
CGAACTA652.3075076E-531.87287329
CGAACAT450.0044556330.89728249
GACGTAA450.004513616730.81573743
TAACGCT450.004560167430.7511837
GTATTAA450.00462057530.6685771
AGTACGG23350.028.3717713
GAGTACG23850.028.06296712
Produced by FastQC (version 0.11.5)