FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755879

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755879
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences651889
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT42630.6539456870724925No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC41060.6298618323058067No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC35340.5421168327736777No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC34160.5240155916114553No Hit
GACCTAGGACGGTGACCTGGGTCCCAGTTCCGAAGACATAAGTGTTAATTCCTGCATAGGAGCAGCAGTAATAAT31080.47676828417107825No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG30510.46802446428763184No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC30330.4652632580086487No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA25320.3884096832436197No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT23570.3615646221979509No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC23390.3588034159189678No Hit
GATTATTACTGCTGCTCCTATGCAGGAATTAACACTTATGTCTTCGGAACTGGGACCCAGGTCACCGTCCTAGGT22780.3494459946401918No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC22490.34499738452405243No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG22470.3446905838263876No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG22320.34238957859390173No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTT21650.3321117552221314No Hit
ACCTAGGACGGTGACCTGGGTCCCAGTTCCGAAGACATAAGTGTTAATTC21400.32827674650132155No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG19780.3034258899904739No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT19660.3015850858044851No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG19350.29682967499068097No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT18420.28256344254926835No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTGGGTCCCAGTTCCGAAGACATAAGTGTTAATTCCTGCATAGGAGCAG18370.2817964408051064No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG16830.2581727870849178No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC16740.2567921839454263No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGAATTATAATATTGTCTCCTGGTACCAACAACACCCAGGCAAAGC16700.25617858255009673No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT15950.24467355638766722No Hit
GTCCTGGGCCCAATCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG15170.23270832917874057No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG14820.22733931696960677No Hit
CCCCTGAGGGCCGCTTACTGACGTCAAAAATCATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA14010.21491388871418293No Hit
GGACACAGGGTCCTGGGCCCAATCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC14010.21491388871418293No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGAATTATAATATTGTCTCCTGGTACCAACAACA13910.21337988522585902No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTGGGTC13820.21199928208636745No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13420.20586326813307174No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA13270.20356226290058582No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC13240.20310206185408866No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA13200.20248846045875907No Hit
GCCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGATTTGGAGCC13150.2017214587145971No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG13150.2017214587145971No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGC12940.19850005138911686No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT12630.19374464057531265No Hit
GTGTTAATTCCTGCATAGGAGCAGCAGTAATAATCAGCCTCGTCCTCAGC12550.19251743778465352No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA12380.18990963185450283No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAATCTGCCCTGACTCAGC12260.1880688276685141No Hit
CCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAATCTGGCAACACGGCCTCCCTGACAATCTCTGGGCTCCAGG12250.18791542731968172No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12180.18684162487785497No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG11700.1794784081339001No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC11350.17410939592476632No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAATC11240.17242199208761No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG11240.17242199208761No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC10740.16475197464599034No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC10650.1633713715064988No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCTCCTAT10470.16061016522751573No Hit
CAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGC10450.16030336452985094No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG10300.15800235929736506No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTG10210.15662175615787352No Hit
GGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTTTGACGTC10170.15600815476254393No Hit
GGCTGAGGACGAGGCTGATTATTACTGCTGCTCCTATGCAGGAATTAACA10060.1543207509253876No Hit
GTCAGGGAGGCCGTGTTGCCAGATTTGGAGCCAGAGAAGCGATTAGAAAC10060.1543207509253876No Hit
GTACCAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTTTGACGTCA9930.1523265463905665No Hit
CAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTTTGACGTCAGTAAGCGGCCCTCAGGGGTTTCTAATCG9830.15079254290224256No Hit
TCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGATTTGGAGCCAGAGAAGCGATTAGAAACC9590.14711093453026514No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA9570.14680413383260033No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC9500.14573033139077357No Hit
CCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT9490.1455769310419412No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT9400.14419632790244966No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAATCTGCCCTGACT9370.14373612685595247No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG9270.14220212336762855No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC9120.13990111813514264No Hit
GGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGC9090.13944091708864545No Hit
CCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACA9030.1385205149956511No Hit
GGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC8880.1362195097631652No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC8870.1360661094143328No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC8680.13315150278651733No Hit
GCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGATTTGGAGCCAGAGAAG8670.13299810243768492No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAG8650.13269130174002017No Hit
GGCAAAGCCCCCAAACTCATGATTTTTGACGTCAGTAAGCGGCCCTCAGG8630.13238450104235536No Hit
GCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCTCCTATGCAGGAA8620.13223110069352298No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGG8520.13069709720519906No Hit
GTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGG8500.13039029650753425No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG8490.13023689615870188No Hit
CTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC8390.12870289267037793No Hit
GCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAATCTGGCAACACGG8310.1274756898797188No Hit
GTATCAACGCAGAGTACGGGGGGGTCACAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTG8210.12594168639139486No Hit
CACCCAGGCAAAGCCCCCAAACTCATGATTTTTGACGTCAGTAAGCGGCCCTCAGGGGTTTCTAATCGCTTCT8190.12563488569373008No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC8170.12532808499606526No Hit
GACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGG8160.1251746846472329No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT8140.12486788394956812No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCT8110.12440768290307093No Hit
CTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTC8070.12379408150774135No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGG8050.12348728081007657No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT8040.12333388046124416No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAATCTGC7930.12164647662408784No Hit
AGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGA7930.12164647662408784No Hit
GTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA7890.12103287522875827No Hit
GTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTT7820.1199590727869315No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAG7730.11857846964743997No Hit
GGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAATCTGCCCTGACTCAGCCTGCCT7690.1179648682521104No Hit
GTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGACAATATTATAA7680.11781146790327801No Hit
GTCCAGGAGACCCAGACACGGAGGCAGGCTGAGTCAGGGCAGATTGGGCC7660.11750466720561323No Hit
GCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCA7630.11704446615911604No Hit
CACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCC7600.11658426511261887No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA7590.11643086476378647No Hit
TTGTCAGGGAGGCCGTGTTGCCAGATTTGGAGCCAGAGAAGCGATTAGAAACCCCTGAGGGCCGCTTACTGACGT7590.11643086476378647No Hit
GCCCCCAAACTCATGATTTTTGACGTCAGTAAGCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAAT7520.11535706232195973No Hit
GGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGT7500.11505026162429494No Hit
CCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAG7490.11489686127546254No Hit
TCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAATCTGCC7470.11459006057779775No Hit
ATATTATAATTCCCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGAT7420.11382305883363578No Hit
GCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCT7330.11244245569414425No Hit
GGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG7280.11167545394998228No Hit
CTCCCTGACAATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCTCCTATGCAGGAATTAACA7270.11152205360114989No Hit
TGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAG7220.11075505185698792No Hit
GCATAGGAGCAGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGA7200.11044825115932312No Hit
CCCTGACAATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGC7180.11014145046165835No Hit
ACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAAC7130.10937444871749638No Hit
GCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGCC7110.10906764801983161No Hit
CATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCC7100.10891424767099919No Hit
CTTCGGAACTGGGACCCAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC7100.10891424767099919No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG7090.10876084732216681No Hit
GAACTGGGACCCAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACT7080.10860744697333442No Hit
TTATAATATTGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAAC7070.10845404662450202No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA7070.10845404662450202No Hit
CAGTAAGCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAATCTGGCAACACGGCCTCCCTGACAATCT7010.10753364453150766No Hit
GCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGG6980.10707344348501048No Hit
CTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTTTG6930.10630644174084852No Hit
GGACGAGGCTGATTATTACTGCTGCTCCTATGCAGGAATTAACACTTATGTCTTCGGAACTGGGACCCAGGTCAC6900.10584624069435135No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG6880.10553943999668655No Hit
GAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC6840.10492583860135699No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACC6790.104158836857195No Hit
AGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAG6670.10231803267120629No Hit
ATTAGAAACCCCTGAGGGCCGCTTACTGACGTCAAAAATCATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTA6660.1021646323223739No Hit
GACAATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCTCCTATGCAGGAATTAACACTTATGT6620.10155103092704433No Hit
GTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGG6610.10139763057821194No Hit
ACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGC6610.10139763057821194No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGGC200.007329625551.7998936
CTCGTAT1050.046.1259439
TCTCGTA1050.046.0904338
TTATACA1200.045.9277882
GTATCAA10150.045.1393931
TCGTATG1150.042.1409640
GTGGTAT1400.041.8303031
CTTATAC1350.040.8278351
TATACAC1350.040.8247033
CGTATGC1200.040.41000741
CCGTCTT1300.040.08033447
TTAACGG350.001342033439.46051835
GGTATCA3950.038.3729931
AGGGGGG1502.0063453E-938.0055970
GCCGTCT1400.037.20306446
TGGTATC1600.036.5987052
ATCAACG12450.036.5208933
TATCAAC12850.035.9201772
TATCTCG1350.035.81227536
GACCGGA1350.035.7298426
Produced by FastQC (version 0.11.5)