FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755880

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755880
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences651889
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT40430.6201976103293659No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC37210.5708026980053352No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC36760.5638996823078776No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC30360.4657234590551459No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC28770.4413328035907954No Hit
GACCTAGGACGGTGACCTGGGTCCCAGTTCCGAAGACATAAGTGTTAATTCCTGCATAGGAGCAGCAGTAATAAT27480.42154415859141664No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG26770.4106527338243167No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC23860.3660132323140903No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG23460.35987721836079456No Hit
GATTATTACTGCTGCTCCTATGCAGGAATTAACACTTATGTCTTCGGAACTGGGACCCAGGTCACCGTCCTAGGT22950.35205380057034247No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTT22630.3471449894077059No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA22550.34591778661704675No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT21940.3365603653382708No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC21790.3342593601057849No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG19060.2923810648745415No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG18710.28701205266540775No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT18480.28348384464226273No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG18470.28333044429343035No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT18440.28287024324693316No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGAATTATAATATTGTCTCCTGGTACCAACAACACCCAGGCAAAGC18310.28087603871211203No Hit
ACCTAGGACGGTGACCTGGGTCCCAGTTCCGAAGACATAAGTGTTAATTC18240.2798022362702853No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT18110.2778080317354642No Hit
GTCCTGGGCCCAATCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG17000.2607805930150685No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG16940.25986019092207413No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTGGGTCCCAGTTCCGAAGACATAAGTGTTAATTCCTGCATAGGAGCAG16840.2583261874337502No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC16270.2495823675503038No Hit
GGACACAGGGTCCTGGGCCCAATCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC15840.24298615255051087No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGAATTATAATATTGTCTCCTGGTACCAACAACA14680.2251917120859533No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG14080.2159876911560097No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14040.21537408976068012No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTGGGTC13330.20448266499358017No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA13310.20417586429591542No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13150.2017214587145971No Hit
CCCCTGAGGGCCGCTTACTGACGTCAAAAATCATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA13150.2017214587145971No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGC13110.20110785731926756No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG13080.20064765627277034No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG12760.19573884511013379No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA12720.1951252437148042No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC12700.1948184430171394No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAATCTGCCCTGACTCAGC12370.18975623150567045No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAATC12320.18898922976150848No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT12270.1882222280173465No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC11980.1837736179012071No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA11970.1836202175523747No Hit
GCCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGATTTGGAGCC11690.1793250077850677No Hit
CCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAATCTGGCAACACGGCCTCCCTGACAATCTCTGGGCTCCAGG11530.17687060220374942No Hit
GTGTTAATTCCTGCATAGGAGCAGCAGTAATAATCAGCCTCGTCCTCAGC11380.1745695969712635No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11280.1730355934829396No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC10570.16214416871583964No Hit
GGCTGAGGACGAGGCTGATTATTACTGCTGCTCCTATGCAGGAATTAACA10290.15784895894853265No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCTCCTAT10260.15738875790203546No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC10210.15662175615787352No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC10110.15508775266954958No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG10070.15447415127421998No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT9950.15263334708823126No Hit
CAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTTTGACGTCAGTAAGCGGCCCTCAGGGGTTTCTAATCG9930.1523265463905665No Hit
CAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGC9750.1495653401115834No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTG9670.14833813732092427No Hit
GTACCAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTTTGACGTCA9640.1478779362744271No Hit
GGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTTTGACGTC9440.14480992929777922No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC9300.1426623244141257No Hit
GGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG9300.1426623244141257No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG9290.1425089240652933No Hit
GGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGC9280.14235552371646093No Hit
GTCAGGGAGGCCGTGTTGCCAGATTTGGAGCCAGAGAAGCGATTAGAAAC9240.14174192232113136No Hit
CCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT9080.13928751673981307No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT9060.1389807160421483No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC8950.13729331220499194No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG8880.1362195097631652No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA8570.131464098949361No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAATCTGCCCTGACT8560.13131069860052863No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA8540.13100389790286382No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC8540.13100389790286382No Hit
CCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACA8520.13069709720519906No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG8480.13008349580986947No Hit
GCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCTCCTATGCAGGAA8380.12854949232154553No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAG8340.12793589092621596No Hit
GTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGG8320.12762909022855118No Hit
TTATAATATTGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAAC8250.12655528778672442No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC8240.12640188743789205No Hit
GTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGACAATATTATAA8180.12548148534489767No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT8150.1250212842984005No Hit
AGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGA8020.12302707976357939No Hit
GCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGATTTGGAGCCAGAGAAG8020.12302707976357939No Hit
GCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCA7950.12195327732175262No Hit
GTCCAGGAGACCCAGACACGGAGGCAGGCTGAGTCAGGGCAGATTGGGCC7940.12179987697292023No Hit
GGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAATCTGCCCTGACTCAGCCTGCCT7920.12149307627525543No Hit
GTATCAACGCAGAGTACGGGGGGGTCACAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTG7900.12118627557759067No Hit
CTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC7850.1204192738334287No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCA7820.1199590727869315No Hit
CTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTC7800.11965227208926674No Hit
GGCAAAGCCCCCAAACTCATGATTTTTGACGTCAGTAAGCGGCCCTCAGG7770.11919207104276956No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGG7730.11857846964743997No Hit
GCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAATCTGGCAACACGG7720.11842506929860758No Hit
TCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGATTTGGAGCCAGAGAAGCGATTAGAAACC7570.11612406406612168No Hit
GACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGG7530.11551046267079211No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAATCTGC7530.11551046267079211No Hit
CACCCAGGCAAAGCCCCCAAACTCATGATTTTTGACGTCAGTAAGCGGCCCTCAGGGGTTTCTAATCGCTTCT7520.11535706232195973No Hit
GTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA7500.11505026162429494No Hit
GCCCCCAAACTCATGATTTTTGACGTCAGTAAGCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAAT7300.11198225464764708No Hit
CCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAG7280.11167545394998228No Hit
GGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGC7280.11167545394998228No Hit
ATATTATAATTCCCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGAT7270.11152205360114989No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCT7250.1112152529034851No Hit
GGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGT7230.11090845220582031No Hit
ACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGC7200.11044825115932312No Hit
GTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTT7190.11029485081049074No Hit
GGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC7160.10983464976399356No Hit
CACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCC7130.10937444871749638No Hit
TCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAATCTGCC7130.10937444871749638No Hit
GCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCT7070.10845404662450202No Hit
TGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAG6940.10645984208968091No Hit
CTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTTTG6910.10599964104318373No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAG6840.10492583860135699No Hit
GAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC6840.10492583860135699No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA6730.10323843476420065No Hit
CTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGC6660.1021646323223739No Hit
CAGTAAGCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAATCTGGCAACACGGCCTCCCTGACAATCT6660.1021646323223739No Hit
CTTCGGAACTGGGACCCAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC6600.10124423022937953No Hit
GGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGG6600.10124423022937953No Hit
GGACGAGGCTGATTATTACTGCTGCTCCTATGCAGGAATTAACACTTATGTCTTCGGAACTGGGACCCAGGTCAC6550.10047722848521759No Hit
GACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGG6540.10032382813638517No Hit
TTGTCAGGGAGGCCGTGTTGCCAGATTTGGAGCCAGAGAAGCGATTAGAAACCCCTGAGGGCCGCTTACTGACGT6540.10032382813638517No Hit
CATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCC6530.1001704277875528No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG352.3009934E-549.179378
GTGGTAT1500.043.7432751
GGTATCA4350.040.488331
GTATCAA9650.038.2917671
TGGTATC1700.036.523432
AAAACCG400.002623152234.4255565
TATCAAC10800.034.174962
ATCAACG11150.033.0539363
TCAACGC11150.033.0361864
CAACGCA11350.032.4540525
AACGCAG11650.031.3228246
CGCTTTT450.004440109730.91828550
CGCAGAG11850.030.794178
AGAGTAC11900.030.66478211
CAGAGTA12500.029.46827510
ACGCAGA12450.029.3101167
TAGACAT605.488593E-428.6879635
TAAGTGC854.4992794E-628.3504585
GCAGAGT13250.027.8002649
AGACATT500.00781443427.540456
Produced by FastQC (version 0.11.5)