FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences948353
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC31900.33637263761489655No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGT29810.3143344303228861No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGTCCCGCTGTGCCACGCCTGACAGTAATAGTCAGCCT26190.2761629899415091No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT25640.27036346170676956No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC24900.26256046008184714No Hit
TCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGGCTGTATTGCCAGAATTGGAGCCAGAGAATCGCTCAGGGATC23670.24959060602961136No Hit
CCTATGCGCTGACTCAGCCACCCTCAGTGTCCGTGTCCGCAGGACAGACA23050.24305295601954124No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG23030.24284206408373252No Hit
GTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC22950.2419984963404977No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG22590.23820244149594083No Hit
GATTACGTGGCCTCCTATGCGCTGACTCAGCCACCCTCAGTGTCCGTGTCCGCAGGACAGACAGCCACCATCAC21840.23029399390311414No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT21780.229661318095688No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG20710.21837859952992186No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC20370.21479343662117378No Hit
GGCTATGGATGAGGCTGACTATTACTGTCAGGCGTGGCACAGCGGGACGGTGTTCGGCGGAGGGACCAAGCTGAC20110.21205184145566047No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC18980.20013644708246825No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18210.1920171075538328No Hit
GTAATAGTCAGCCTCATCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGG18110.19096264787478925No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG17730.1869557010944237No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17290.18231607850663203No Hit
CCCTCAGTGTCCGTGTCCGCAGGACAGACAGCCACCATCACCTGCTCTGG17110.1804180510843536No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17010.17936359140531002No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT16970.17894180753369263No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16450.1734586172026661No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16410.1730368333310487No Hit
GACTATTACTGTCAGGCGTGGCACAGCGGGACGGTGTTCGGCGGAGGGAC16340.17229871155571816No Hit
GCATCGGAGGTGCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGATTACGTGG15990.1686081026790657No Hit
CTATTACTGTCAGGCGTGGCACAGCGGGACGGTGTTCGGCGGAGGGACCA15840.16702641316050038No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15140.15964519540719543No Hit
GCCGAACACCGTCCCGCTGTGCCACGCCTGACAGTAATAGTCAGCCTCATCCATAGCCTGGGTCCCGCTGATGGT14780.1558491405626386No Hit
GAGTGGCTGTATTGCCAGAATTGGAGCCAGAGAATCGCTCAGGGATCCCT14440.1522639776538905No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG14440.1522639776538905No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC14410.15194763975017742No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14160.1493114905525685No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14130.14899515264885543No Hit
CAGTAATAGTCAGCCTCATCCATAGCCTGGGTCCCGCTGATGGTCAGAGT13940.14699167925867265No Hit
GCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGATTACGTGGCCTCCTATGCGCTGACT13900.14656989538705525No Hit
GGACAGACAGCCACCATCACCTGCTCTGGACATAAATTGGGTGAAAAATA13850.14604266554753345No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG13620.14361740828573327No Hit
GTTTTGGTCATCTATCAAGATTACAACCGACCCTCAGGGATCCCTGAGCG13280.14003224537698516No Hit
GTGCCACGCCTGACAGTAATAGTCAGCCTCATCCATAGCCTGGGTCCCGC13210.13929412360165466No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13190.13908323166584594No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC12860.13560351472500218No Hit
GATTACAACCGACCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAATTC12760.13454905504595863No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12670.13360004133481942No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC12620.13307281149529765No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT12510.13191290584834972No Hit
GTGTCCGTGTCCGCAGGACAGACAGCCACCATCACCTGCTCTGGACATAA12110.12769506713217546No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA11700.12337178244809686No Hit
ACAGAAGCCCGGCCAGTCCCCTGTTTTGGTCATCTATCAAGATTACAACCGACCCTCAGGGATCCCTGAGCGATT11450.12073563325048794No Hit
CCCCTGTTTTGGTCATCTATCAAGATTACAACCGACCCTCAGGGATCCCT11290.11904849776401824No Hit
GGACATAAATTGGGTGAAAAATATGCTTATTGGTATCAACAGAAGCCCGGCCAGTCCCCTGTTTTGGTCATCTAT11140.11746680824545291No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10970.11567422679107885No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10960.11556878082317448No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGTCCCGCTGTGCC10920.11514699695155707No Hit
GTTGTAATCTTGATAGATGACCAAAACAGGGGACTGGCCGGGCTTCTGTTGATACCAATAAGCATATTTTTCACC10900.11493610501574836No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC10890.114830659047844No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10860.11451432114413093No Hit
GCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGATTACGTGGCCTCCTATGCG10680.11261629372185253No Hit
GTATCAACGCAGAGTACGGGGGGCCTCAGGAAGCAGCATCGGAGGTGCCTCAGCCATGGCATGGATCCCTCTCTT10670.11251084775394815No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC10510.11082371226747846No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGTCC10350.10913657678100876No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC10310.10871479290939133No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG10210.10766033323034777No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10170.10723854935873035No Hit
GTCCCCTGTTTTGGTCATCTATCAAGATTACAACCGACCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAATT10110.10660587355130421No Hit
GCCTCATCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGGCTGTATTGCC9940.10481329209693015No Hit
CACCTGCTCTGGACATAAATTGGGTGAAAAATATGCTTATTGGTATCAAC9740.10270437273884303No Hit
CAGCCACTCTGACCATCAGCGGGACCCAGGCTATGGATGAGGCTGACTATTACTGTCAGGCGTGGCACAGCGGGA9680.10207169693141689No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC9560.10080634531656461No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTACGC200.007399555851.6774716
GTGGTAT1850.050.2834361
TCGTATG605.498805E-946.10326840
CGTATGC704.311005E-1044.48026341
ATCAACG29500.037.018973
TCAACGC29700.036.653694
CAACGCA29900.036.5237355
AACGCAG30650.035.517616
TCTCGTA709.640062E-734.5427238
CGCAGAG33550.032.3431438
CAGAGTA33700.032.30310410
AGAGTAC34100.031.82315811
ACGCAGA34650.031.4157947
GCAGAGT35600.030.5774489
GTATCAA39350.029.856751
ATGCCGT953.0599767E-729.20440344
TATACAC1302.0008883E-1029.1498573
CTCGTAT953.1536547E-729.1039939
ACGGGTA1202.7248461E-928.70970716
ATCTCCG1103.7140126E-828.18622810
Produced by FastQC (version 0.11.5)