FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755882

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755882
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences948353
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC31490.33204935293081794No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGT25820.2722614891290479No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24100.25412478264949867No Hit
GTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC23180.24442375360229787No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23100.24358018585906305No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG22980.24231483424421077No Hit
CCTATGCGCTGACTCAGCCACCCTCAGTGTCCGTGTCCGCAGGACAGACA22970.24220938827630636No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGTCCCGCTGTGCCACGCCTGACAGTAATAGTCAGCCT22920.24168215843678462No Hit
TCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGGCTGTATTGCCAGAATTGGAGCCAGAGAATCGCTCAGGGATC21910.23103211567844462No Hit
GATTACGTGGCCTCCTATGCGCTGACTCAGCCACCCTCAGTGTCCGTGTCCGCAGGACAGACAGCCACCATCAC21870.23061033180682722No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG21060.22206920840657432No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20350.21458254468536506No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG20180.212789963230991No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19540.20604142128511216No Hit
GGCTATGGATGAGGCTGACTATTACTGTCAGGCGTGGCACAGCGGGACGGTGTTCGGCGGAGGGACCAAGCTGAC19220.20266715031217278No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC18410.19412602691191994No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC17560.18516311964004964No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG16890.17809823979045777No Hit
GTAATAGTCAGCCTCATCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGG16810.17725467204722292No Hit
CCCTCAGTGTCCGTGTCCGCAGGACAGACAGCCACCATCACCTGCTCTGG16760.17672744220770115No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16620.17525119865704014No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT15910.16776453493583085No Hit
GACTATTACTGTCAGGCGTGGCACAGCGGGACGGTGTTCGGCGGAGGGAC15880.16744819703211777No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT15840.16702641316050038No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15830.166920967192596No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15340.16175411476528254No Hit
GCATCGGAGGTGCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGATTACGTGG15140.15964519540719543No Hit
CTATTACTGTCAGGCGTGGCACAGCGGGACGGTGTTCGGCGGAGGGACCA14920.15732538411329958No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG14590.15384566717245582No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14470.15258031555760354No Hit
GCCGAACACCGTCCCGCTGTGCCACGCCTGACAGTAATAGTCAGCCTCATCCATAGCCTGGGTCCCGCTGATGGT14430.15215853168598611No Hit
GGACAGACAGCCACCATCACCTGCTCTGGACATAAATTGGGTGAAAAATA14320.15099862603903821No Hit
GCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGATTACGTGGCCTCCTATGCGCTGACT14120.14888970668095108No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13920.14678078732286395No Hit
GAGTGGCTGTATTGCCAGAATTGGAGCCAGAGAATCGCTCAGGGATCCCT13720.14467186796477682No Hit
GTTTTGGTCATCTATCAAGATTACAACCGACCCTCAGGGATCCCTGAGCG13570.1430901784462115No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13500.142352056670881No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG13450.1418248268313592No Hit
GATTACAACCGACCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAATTC13140.13855600182632416No Hit
GGACATAAATTGGGTGAAAAATATGCTTATTGGTATCAACAGAAGCCCGGCCAGTCCCCTGTTTTGGTCATCTAT13070.13781788005099366No Hit
CAGTAATAGTCAGCCTCATCCATAGCCTGGGTCCCGCTGATGGTCAGAGT12990.1369743123077588No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC12670.13360004133481942No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC12490.13170201391254102No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12260.12927675665074082No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12260.12927675665074082No Hit
GTTGTAATCTTGATAGATGACCAAAACAGGGGACTGGCCGGGCTTCTGTTGATACCAATAAGCATATTTTTCACC12030.1268514993889406No Hit
GTGCCACGCCTGACAGTAATAGTCAGCCTCATCCATAGCCTGGGTCCCGC11890.12537525583827963No Hit
GTGTCCGTGTCCGCAGGACAGACAGCCACCATCACCTGCTCTGGACATAA11690.1232663364801925No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT11680.12316089051228815No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC11520.12147375502581845No Hit
GTATCAACGCAGAGTACGGGGGGCCTCAGGAAGCAGCATCGGAGGTGCCTCAGCCATGGCATGGATCCCTCTCTT11290.11904849776401824No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT11200.11809948405287905No Hit
ACAGAAGCCCGGCCAGTCCCCTGTTTTGGTCATCTATCAAGATTACAACCGACCCTCAGGGATCCCTGAGCGATT11150.11757225421335725No Hit
CACCTGCTCTGGACATAAATTGGGTGAAAAATATGCTTATTGGTATCAAC10810.11398709130460916No Hit
CCCCTGTTTTGGTCATCTATCAAGATTACAACCGACCCTCAGGGATCCCT10740.11324896952927867No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10570.1114563880749046No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10480.11050737436376538No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10060.10607864371178244No Hit
GCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGATTACGTGGCCTCCTATGCG9960.10502418403273886No Hit
GTCCCCTGTTTTGGTCATCTATCAAGATTACAACCGACCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAATT9890.10428606225740837No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA9800.10333704854626916No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGTCC9680.10207169693141689No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTA452.3753528E-456.06878770
GTGGTAT2650.046.863581
ATCAACG32450.036.4849243
TCAACGC32900.036.0733764
CAACGCA33350.035.5866285
AACGCAG34150.034.7529756
CGCAGAG36500.032.3269588
AGAGTAC37700.031.48047611
ACGCAGA38200.031.068437
TAACGAC450.0043374531.06790254
GGTAACG450.00437166731.01805152
CAGAGTA38500.030.91569110
GCAGAGT39050.030.3921629
GTATCAA41800.029.7926921
AGTACTT9250.028.26411813
GAGTACT9600.027.59199312
CAACGTT756.9099717E-427.58110869
TATCAAC45600.027.0472642
TAACGGC1950.026.53459736
TTAACGG1950.026.52898635
Produced by FastQC (version 0.11.5)