FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755883

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755883
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences903494
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAACTGGCCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT43280.47902919111803727No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37050.4100746656867671No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC26180.28976396080106787No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26130.28921055369487786No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23720.26253633117652136No Hit
GTTATTATACTGCTGACAGTAATAAATTGCTAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT21560.23862914418911468No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20950.23187757749359708No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20920.23154553322988308No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20560.22756100206531532No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT20340.22512601079807945No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19820.2193705768937038No Hit
GTCCCAGACCCACTGCCACTGAACCGGGCTGGGATACCAGTGGCCCTGGT19610.2170462670477059No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTAGCAATTTATTACTGTCAGCAGTATAATAACTGG18470.2044285850265746No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17760.19657020411867704No Hit
CTGGTATCCCAGCCCGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT17130.18959727458068343No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16850.18649819478601962No Hit
CTCCTGCAGGGCCAGTCAGAGTATTGCCAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGCCTCCCAGGCT16650.18428456636125973No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16170.17897185814183603No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16130.17852913245688407No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15920.17620482261088619No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15740.1742125570286023No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15400.1704493887065105No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15310.16945325591536856No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTGCCAG15100.16712894606937068No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCCGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA15080.1669075832268947No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCACGGAGGCCAGTTATTATACTG15010.16613281327822874No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14750.1632550963260409No Hit
GAGTATTGCCAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGCCTCCCAGGCTCCTCATCTATGGTGCATC14720.16292305206232693No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14560.161152149322519No Hit
CCATAGATGAGGAGCCTGGGAGGCTGGCCAGGTTTCTGCTGGTACCAGGC14310.15838511379156917No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTGCCAGCAACTTAGCCTGGTA14280.1580530695278552No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC13800.1527403613084315No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC13760.15229763562347953No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCGGGCTGGGATACCA12710.14067608639349016No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12210.13514201533159048No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCACCATCAGCAGCC12180.13480997106787648No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11790.13049339563959472No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11600.12839044863607285No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT11470.12695159015997892No Hit
CTGCTACTCTGGCTCCCAGTTTCAGATGTTACTGGAGAAAGAGTGATGAC11450.12673022731750294No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10710.1185398021458914No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10390.1149979966662756No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9970.11034937697427985No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9920.10979596986808988No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9890.1094639256043759No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9710.10747166002209202No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9490.10503666875485615No Hit
GGAGAAAGAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG9420.1042618988061902No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9210.10193758896019232No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7450.043.8443641
GTATCAA19900.042.676651
GTGGTAT1950.037.0280761
TATCAAC23450.036.6558762
ATCAACG23600.036.4228943
TCAACGC23800.036.1168174
CAACGCA24250.035.5883945
AACGCAG24750.034.7285966
AGAGTAC26850.031.75453611
CGCAGAG27100.031.5884578
ACGCAGA27700.030.7801197
CGATTGT1003.716438E-430.66147670
GCAGAGT28650.029.9994839
TAATACG605.52993E-428.6526762
TCGTATG756.0124694E-527.69974940
TGTTAGA9000.025.89191470
AGTACGG23300.025.67389313
GAGTACG23500.025.30909512
AGTACTT10350.024.91261513
CGTTCGG14700.024.80020124
Produced by FastQC (version 0.11.5)