FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755884

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755884
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences903494
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAACTGGCCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT44600.49363913872145254No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36080.3993385678266817No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC25610.2834551197905022No Hit
GTTATTATACTGCTGACAGTAATAAATTGCTAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT22890.2533497732137679No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21670.23984663982273263No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21600.2390718698740667No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21090.233427117390929No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20550.2274503206440773No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19430.21505400146542203No Hit
GTCCCAGACCCACTGCCACTGAACCGGGCTGGGATACCAGTGGCCCTGGT19390.21461127578047004No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT19310.2137258244105661No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTAGCAATTTATTACTGTCAGCAGTATAATAACTGG19040.21073742603714027No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17300.19147885874172935No Hit
CTGGTATCCCAGCCCGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT16760.18550206199487765No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCACGGAGGCCAGTTATTATACTG15820.17509800839850626No Hit
CTCCTGCAGGGCCAGTCAGAGTATTGCCAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGCCTCCCAGGCT15770.17454460129231628No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15770.17454460129231628No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15280.16912121165165458No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15020.16624349469946673No Hit
GAGTATTGCCAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGCCTCCCAGGCTCCTCATCTATGGTGCATC14940.16535804332956278No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14850.16436191053842084No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14610.16170555642870899No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCCGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA14520.16070942363756704No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14260.15783170668537921No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14250.1577210252641412No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTGCCAG14100.1560608039455713No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14070.15572875968185731No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13800.1527403613084315No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13730.15196559135976553No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTGCCAGCAACTTAGCCTGGTA13720.15185490993852752No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC13300.1472062902465318No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13290.1470956088252938No Hit
CCATAGATGAGGAGCCTGGGAGGCTGGCCAGGTTTCTGCTGGTACCAGGC13110.1451033432430099No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCACCATCAGCAGCC12700.14056540497225217No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCGGGCTGGGATACCA12640.1399013164448242No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12280.13591678528025644No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC12020.13303906832806858No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11880.13148952843073666No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT11610.12850113005731084No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10990.12163888194055522No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10550.1167688994060835No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10520.11643685514236951No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10460.11577276661494154No Hit
CTGCTACTCTGGCTCCCAGTTTCAGATGTTACTGGAGAAAGAGTGATGAC10380.1148873152450376No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10100.11178823545037378No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9190.10171622611771633No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)