FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755885

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755885
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences556102
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6130.1102315762216284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21800.042.7023431
GGTATCA7800.039.34051
GTGGTAT3700.036.3418771
TATCAAC26350.034.936152
TCAACGC27500.033.6005634
ATCAACG27600.033.478823
CAACGCA28000.033.1236885
AACGCAG29100.031.871596
TGGTATC4250.030.0163042
ACGCAGA31750.029.2009377
CGCAGAG32200.028.7928498
CAGAGTA32950.028.45127510
AGAGTAC32500.028.42101911
GCAGAGT33550.028.0451859
ACGAGAC1503.45608E-1127.58006922
TACGTGA600.007795055427.55281369
CGATTAA658.012538E-426.88930157
CGAACTA1306.2991603E-926.53600329
GCTTACC658.6834555E-426.5216831
TACGACT700.00133790224.6272774
Produced by FastQC (version 0.11.5)