FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1479566
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT64130.43343791355032485No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44700.302115620391385No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG41650.2815014673221742No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC41470.2802848943541552No Hit
GTATCAACGCAGAGTACGGGAATTAGGACTCCTCAGGTCACCTTCTCACA41010.27717587454699555No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT37940.25642654670356035No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA37180.25128990528303574No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34950.23621791795702252No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34650.2341902963436575No Hit
GTCCCAGACAGATTCAGTGGCAGTGGGGCAGGGACAGATTTCACACTAAA34410.23256819905296552No Hit
GTTTATTACTGCATGCAAGCAACACAATTTCCTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA33580.22695844592265568No Hit
GTGATGGAAACACCTTCTTGAGTTGGCTTCAGCAGAGGCCAGGCCAGCCTCCAAGACTCCTAATTTATAAGATTT33540.22668809637420703No Hit
GAATTAGGACTCCTCAGGTCACCTTCTCACAATGAGGCTCCTTGCTCAGCTTCTGGGGCTGCTAATGCTCTGGGT33060.22344390179282306No Hit
GGCCAGGCCAGCCTCCAAGACTCCTAATTTATAAGATTTCTAACCGGTTC30220.20424908385296767No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29700.20073453972313504No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT29190.19728758298041454No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG28610.19336751452790887No Hit
GTCTGGGACCCCAGAGAACCGGTTAGAAATCTTATAAATTAGGAGTCTTGGAGGCTGGCCTGGCCTCTGCTGAAG26370.17822793981478352No Hit
GTAATAAACCCCGACATCCTCAGCTTCCACCCTGCTGATTTTTAGTGTGAAATCTGTCCCTGCCCCACTGCCA25760.17410510920094135No Hit
TTATTACTGCATGCAAGCAACACAATTTCCTTACACTTTTGGCCAGGGGA25070.16944157949020186No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24450.1652511614892475No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23960.16193937952075135No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23960.16193937952075135No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC23120.15626203900332936No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22890.15470752909974952No Hit
CCTTCTCACAATGAGGCTCCTTGCTCAGCTTCTGGGGCTGCTAATGCTCT22400.15139574713125334No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22260.15044952371168302No Hit
CCTCAGGTCACCTTCTCACAATGAGGCTCCTTGCTCAGCTTCTGGGGCTG21860.14774602822719635No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21700.1466646300334017No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20750.14024382825774587No Hit
CTCCTCACCTGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTA20430.13808103187015652No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20400.13787826970882003No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20070.13564788593411853No Hit
CTCGTACACAGTGATGGAAACACCTTCTTGAGTTGGCTTCAGCAGAGGCC19550.13213334180428585No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA19480.13166023009450067No Hit
AATTAGGACTCCTCAGGTCACCTTCTCACAATGAGGCTCCTTGCTCAGCTTCTGGGGCTGCTAATGCTCTGGGTC19210.1298353706424722No Hit
GCTAATGCTCTGGGTCCCTGGATCCAGTGGGGATGTTGTGATGACCCAGA19150.12942984631979917No Hit
TTCCATCACTGTGTACGAGGCTTTGACTAGACCTGCAGGAGATGGAGGCC18950.12807809857755584No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18810.12713187515798552No Hit
GTTCTCTGGGGTCCCAGACAGATTCAGTGGCAGTGGGGCAGGGACAGATT18710.12645600128686385No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18250.12334698147970417No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18110.12240075806013385No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA17690.11956208780142284No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17690.11956208780142284No Hit
GGCCTGGCCTCTGCTGAAGCCAACTCAAGAAGGTGTTTCCATCACTGTGT17470.11807516528495518No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17450.11793999051073085No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG17450.11793999051073085No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17260.11665583015559967No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC17240.11652065538137535No Hit
CAATGAGGCTCCTTGCTCAGCTTCTGGGGCTGCTAATGCTCTGGGTCCCTGGATCCAGTGGGGATGTTGTGATGA17100.11557443196180502No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17090.11550684457469285No Hit
GGCCAAAAGTGTAAGGAAATTGTGTTGCTTGCATGCAGTAATAAACCCCG16920.11435785899378602No Hit
CTCCAAGACTCCTAATTTATAAGATTTCTAACCGGTTCTCTGGGGTCCCA16620.11233023738042101No Hit
GTCTTGGAGGCTGGCCTGGCCTCTGCTGAAGCCAACTCAAGAAGGTGTTTCCATCACTGTGTACGAGGCTTTGA16390.11077572747684118No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16310.11023502837994385No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA16120.10895086802481269No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16060.10854534370213968No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16050.10847775631502753No Hit
GGTATCAACGCAGAGTACGGGAATTAGGACTCCTCAGGTCACCTTCTCAC15990.10807223199235452No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15590.10536873650786785No Hit
GTACGGGAATTAGGACTCCTCAGGTCACCTTCTCACAATGAGGCTCCTTGCTCAGCTTCTGGGGCTGCTAATGCT15490.10469286263674617No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15360.10381422660428802No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14950.10104314373268918No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC6800.046.000022
GGTATCA22800.043.7208561
GTGGTAT7250.043.1448481
CGTATGC1350.041.0471741
GTATCAA58000.040.9520451
CTAGCGC2300.037.40817628
TATCAAC65950.036.0675662
TCGTATG1350.035.89182740
ATCAACG71950.033.3942683
TATACAC3400.033.362653
ACTAGCG2700.033.13190527
TCAACGC72950.032.8893784
CAACGCA74400.032.340795
AACGCAG75150.032.0637666
CTTATAC3350.031.8084431
CGAGACT2600.031.73594923
TAGCGCT2750.031.2942529
ACGAGAC2700.030.55641222
ACGCAGA80650.029.8264627
CGCAGAG81550.029.4962948
Produced by FastQC (version 0.11.5)