FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1479566
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT56460.3815983876352931No Hit
GTATCAACGCAGAGTACGGGAATTAGGACTCCTCAGGTCACCTTCTCACA41760.28224492858040806No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC41020.2772434619341077No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT36630.24757259899186654No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34160.23087851437516138No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33930.22932400447158155No Hit
GTTTATTACTGCATGCAAGCAACACAATTTCCTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA32700.221010755856785No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31290.21148093427396952No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31140.21046712346728702No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA30830.20837191446680986No Hit
GTCCCAGACAGATTCAGTGGCAGTGGGGCAGGGACAGATTTCACACTAAA30230.20431667124007985No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG30160.2038435595302947No Hit
GTAATAAACCCCGACATCCTCAGCTTCCACCCTGCTGATTTTTAGTGTGAAATCTGTCCCTGCCCCACTGCCA30110.20350562259473384No Hit
GTGATGGAAACACCTTCTTGAGTTGGCTTCAGCAGAGGCCAGGCCAGCCTCCAAGACTCCTAATTTATAAGATTT29040.19627377217373201No Hit
GAATTAGGACTCCTCAGGTCACCTTCTCACAATGAGGCTCCTTGCTCAGCTTCTGGGGCTGCTAATGCTCTGGGT28520.19275922804389936No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27900.18856881004294504No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC27850.1882308731073842No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT26340.17802517765344703No Hit
GGCCAGGCCAGCCTCCAAGACTCCTAATTTATAAGATTTCTAACCGGTTC25420.1718071380391277No Hit
GTCTGGGACCCCAGAGAACCGGTTAGAAATCTTATAAATTAGGAGTCTTGGAGGCTGGCCTGGCCTCTGCTGAAG25210.17038780290977218No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25070.16944157949020186No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24750.16727878310261254No Hit
TTATTACTGCATGCAAGCAACACAATTTCCTTACACTTTTGGCCAGGGGA24310.1643049380696772No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23420.15828966061669436No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23200.15680273810022669No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22380.15126057235702903No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21960.14842190209831801No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC21230.14348802283912987No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21220.14342043545201769No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20570.13902725528972684No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20210.13659410935368885No Hit
CCTTCTCACAATGAGGCTCCTTGCTCAGCTTCTGGGGCTGCTAATGCTCT20010.1352423616114455No Hit
CCTCAGGTCACCTTCTCACAATGAGGCTCCTTGCTCAGCTTCTGGGGCTG19750.13348508954652918No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19640.13274162828829536No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA19620.13260645351407102No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19070.12888914722290187No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18580.1255773652544057No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18250.12334698147970417No Hit
AATTAGGACTCCTCAGGTCACCTTCTCACAATGAGGCTCCTTGCTCAGCTTCTGGGGCTGCTAATGCTCTGGGTC18210.12307663193125552No Hit
GGCCTGGCCTCTGCTGAAGCCAACTCAAGAAGGTGTTTCCATCACTGTGT17480.11814275267206735No Hit
CTCGTACACAGTGATGGAAACACCTTCTTGAGTTGGCTTCAGCAGAGGCC17470.11807516528495518No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16960.1146282085422347No Hit
CTCCTCACCTGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTA16830.1137495725097765No Hit
GGCCAAAAGTGTAAGGAAATTGTGTTGCTTGCATGCAGTAATAAACCCCG16820.11368198512266435No Hit
GCTAATGCTCTGGGTCCCTGGATCCAGTGGGGATGTTGTGATGACCCAGA16730.11307369863865484No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16720.11300611125154268No Hit
TTCCATCACTGTGTACGAGGCTTTGACTAGACCTGCAGGAGATGGAGGCC16390.11077572747684118No Hit
CAGTAATAAACCCCGACATCCTCAGCTTCCACCCTGCTGATTTTTAGTGTGAAATCTGTCCCTGCCCCACTGCCA16060.10854534370213968No Hit
GTTCTCTGGGGTCCCAGACAGATTCAGTGGCAGTGGGGCAGGGACAGATT15930.10766670766968152No Hit
GTCTTGGAGGCTGGCCTGGCCTCTGCTGAAGCCAACTCAAGAAGGTGTTTCCATCACTGTGTACGAGGCTTTGA15550.10509838695941917No Hit
GGGTGGAAGCTGAGGATGTCGGGGTTTATTACTGCATGCAAGCAACACAATTTCCTTACACTTTTGGCCAGGGGA15490.10469286263674617No Hit
GGTATCAACGCAGAGTACGGGAATTAGGACTCCTCAGGTCACCTTCTCAC15350.10374663921717585No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15190.10266524102338119No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15040.10165143021669867No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14940.100975556345577No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)