FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences789095
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATACTACTCCTCGTACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT34300.4346751658545549No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29540.3743528979400452No Hit
GTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT24950.3161849967367681No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19620.2486392639669495No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19100.24204943637965012No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17350.21987213199931568No Hit
GTTTTATACAACTCCAACAATAAGAACTTCTTAGCTTGGTACCAGCAGAA16510.20922702589675515No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15970.20238374340225193No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15290.193766276557322No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14210.1800797115683156No Hit
ATACTACTCCTCGTACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA14210.1800797115683156No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTA13400.16981478782656081No Hit
ACTTAGGAGGCTGTCCTGCTTTCTGCTGGTACCAAGCTAAGAAGTTCTTATTGTTGGAGTTGTATAAAACACT13260.16804060347613406No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13080.16575950931129965No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12990.16461896222888245No Hit
GAGTAGTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT12420.15739549737357353No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12070.15296003649750664No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCTCAGACCCGCTGCCA10960.1388932891476945No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGAGACAGATTTCACTCTCAC10770.1364854675292582No Hit
GTTTATTACTGTCAGCAATATTATACTACTCCTCGTACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA10760.1363587400756563No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10710.13572510280764674No Hit
ATTCAGTGGCAGCGGGTCTGAGACAGATTTCACTCTCACCATCAGCAGCC10560.1338241910036181No Hit
CTCCTAAGTTGCTCATTTACTGGGCTTCTACCCGGGAATCCGGGGTCCCT10440.13230346156039513No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9790.12406617707627092No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9760.1236859947154652No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9370.11874362402499065No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9330.118236714210583No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9240.11709616712816581No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9120.11557543768494288No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG8760.11101324935527408No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC8640.10949251991205114No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCTCAGACCCGCTGCCACTGAATCGGTCAGGGACC8580.10873215519043967No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8380.10619760611840146No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8290.10505705903598427No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGC8250.10455014922157661No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8200.10391651195356705No Hit
GTCTCCAGACTCCCTGGCCGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA8010.10150869033513076No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC7970.10100178052072312No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7930.10049487070631546No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29200.042.42311
GGTATCA11300.041.7181321
GTGGTAT4000.039.571321
TAGCGGC654.6109562E-737.9534764
TATCAAC34050.036.3619772
TGGTATC4450.036.3245242
GTTGCGC400.002432380634.9605158
TCAACGC35600.034.5373544
ATCAACG35600.034.372733
CAACGCA36600.033.768545
AACGCAG37500.033.3242956
GCGCAAA800.003315865532.81544570
AATAGCG751.4541474E-632.77149262
CGCAGAG40600.030.6952698
ATCGTAC450.00472661430.52642415
ACGCAGA40950.030.5167567
AGAGTAC41500.030.27776511
CGTTTAG604.847688E-429.30394263
GCAGAGT42750.029.2318389
CAGAGTA43400.028.95224210
Produced by FastQC (version 0.11.5)