FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2285222
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT77820.34053584290716615No Hit
GTATTACTGTCAGCAGTATGGTAGCTCACCGTTCACTTTTGGCCGGGGGA75720.3313463637230869No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA61340.2684203110244869No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCTCAGACCCACTGCCA55680.24365247665215894No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC48290.21131426180913715No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT47320.20706959761458624No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC47290.20693831934052798No Hit
CATCTATGGTGCGTCCGTGAGGGCCACTGGCATCCCAAGCAGGTTCAGTGGCAGTGGGTCTGAGACAGACTTCA45340.19840523152674008No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG45120.19744252418364608No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG43630.19092236990541836No Hit
GTATGGTAGCTCACCGTTCACTTTTGGCCGGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT43360.1897408654388939No Hit
TCTATGGTGCGTCCGTGAGGGCCACTGGCATCCCAAGCAGGTTCAGTGGCAGTGGGTCTGAGACAGACTTCACT41950.18357078655815495No Hit
CTCCTGCAGGGCCAGTCACTTCGTTAGCAGCAGAAACTTAGCCTGGTACC41940.18352702713346886No Hit
GTCTGAGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT41430.1812952964744782No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT38720.16943649238454733No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAGAGGGCCACCCTCTCCTGCAGGGCCAGTCACTTCGTTAGCAG38460.16829874734270894No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC37430.16379152660004148No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCCGGCCAAAAGTG37370.16352897005192493No Hit
GTGTATTACTGTCAGCAGTATGGTAGCTCACCGTTCACTTTTGGCCGGGGGACCAAGCTGGAGATCAAACGAA37210.16282881925694745No Hit
GTCTGAGACCTGAGGACACGGCCTTATATTTTTGTGTAGGAAGACGCTACGATTTTTGGACTGCCTACTTTGACT36960.16173483363979516No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTTTCTGCTGCTAACGAAGTGA35620.15587107073185888No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG34060.14904460048082857No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT33530.1467253509724657No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCTCAGACCCACTGCCA33200.14528128995782466No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT33030.1445373797381611No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC32920.1440560260666141No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT32640.14283076217540353No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT32610.14269948390134524No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAGAGGGCCACCCTCTCCTGC31890.13954880532394665No Hit
GAGTTGGGACTGAGCTGGATTTTCCTTTTGGCTATTTTAAAAGGTGTCAAGTGTGAAGTGCAGCTGGTAGAAT31520.13792970661056125No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC31090.1360480513490593No Hit
CTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGT30220.1322409814013693No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29290.12817135490556278No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29010.12694609101435222No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC28950.12668353446623565No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG28720.12567706769845555No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGACTGAGCTGGATTTTC28530.1248456386294198No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT28390.12423300668381453No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27460.12016338018800798No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26670.11670638563780675No Hit
GTCTTTGTCTCCAGGGGAGAGGGCCACCCTCTCCTGCAGGGCCAGTCACT26650.11661886678843456No Hit
CTCCAGGCCCTTCCCTGGAGCTTGCCGGACCCAGTGCATGGCAAAGTCAT26100.11421209843069952No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT25160.11009871251020688No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC24720.10817329782401884No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG24480.10712307163155264No Hit
GTATCAACGCAGAGTACGGGAGGTCTGGGAGAGGAGCCCCAGCCCTGAGATTCCCAGGTGTTTCCATTCAGTGAT24230.10602908601440035No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG23720.10379735535540967No Hit
CTCCAGGGAAGGGCCTGGAGTGGGTCTCAGGCATATCTTGGAATAGTGAT23620.10335976110854876No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23470.10270336973825736No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA23410.10244081319014083No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23320.102046978367966No Hit
CACCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAG22990.10060291735332497No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25350.044.25391
GTATCAA69100.042.6292421
TATCAAC77850.037.6168862
ATCAACG82950.035.4285473
TCAACGC83750.034.925774
CAACGCA85700.034.2113885
AACGCAG87700.033.43126
GTCGTAT751.6441427E-632.26487739
TGTCGTA751.6512131E-632.24715438
CGCAGAG94600.031.0230338
AGAGTAC95500.030.69464311
TATACAC4650.030.34193
ACGAGAC4200.030.32011422
CTTATAC5000.030.2826981
AACTGTG40500.030.1648770
GCAGAGT97850.029.9926349
ACGCAGA98450.029.844797
CAGAGTA102400.028.62635410
CGTATGC2700.028.19626441
TCGTATG2250.027.66899540
Produced by FastQC (version 0.11.5)