FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755892

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755892
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2285222
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT72900.319006205961609No Hit
GTATTACTGTCAGCAGTATGGTAGCTCACCGTTCACTTTTGGCCGGGGGA69940.3060534162545258No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA54540.23866390223794448No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCTCAGACCCACTGCCA53480.2340254032212188No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT47080.20601937142212004No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT45950.20107455643259167No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC44140.19315410056440907No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43720.1913162047275932No Hit
GTATGGTAGCTCACCGTTCACTTTTGGCCGGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT40630.17779454249959084No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG40220.1760004060874611No Hit
CATCTATGGTGCGTCCGTGAGGGCCACTGGCATCCCAAGCAGGTTCAGTGGCAGTGGGTCTGAGACAGACTTCA39430.17254341153725983No Hit
GTCTGAGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT39360.17223709556445718No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC38330.16772987482178975No Hit
CTCCTGCAGGGCCAGTCACTTCGTTAGCAGCAGAAACTTAGCCTGGTACC37220.16287257868163355No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT36610.16020325377578196No Hit
TCTATGGTGCGTCCGTGAGGGCCACTGGCATCCCAAGCAGGTTCAGTGGCAGTGGGTCTGAGACAGACTTCACT36130.15810280139084956No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35490.15530219821093968No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCCGGCCAAAAGTG35330.1546020474159622No Hit
GTGTATTACTGTCAGCAGTATGGTAGCTCACCGTTCACTTTTGGCCGGGGGACCAAGCTGGAGATCAAACGAA35300.15447076914190394No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT34120.14930715702894512No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT33470.14646279442434915No Hit
GTCTGAGACCTGAGGACACGGCCTTATATTTTTGTGTAGGAAGACGCTACGATTTTTGGACTGCCTACTTTGACT33090.14479993628627766No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAGAGGGCCACCCTCTCCTGCAGGGCCAGTCACTTCGTTAGCAG31990.13998639957080755No Hit
GAGTTGGGACTGAGCTGGATTTTCCTTTTGGCTATTTTAAAAGGTGTCAAGTGTGAAGTGCAGCTGGTAGAAT31740.13889241395365526No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31450.1376233906377586No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTTTCTGCTGCTAACGAAGTGA31360.1372295558155838No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCTCAGACCCACTGCCA30160.13197842485325278No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC30060.13154083060639185No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC29940.13101571751015875No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT29390.1286089491524237No Hit
CTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGT29290.12817135490556278No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAGAGGGCCACCCTCTCCTGC28900.1264647373428052No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC28150.12318278049134833No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27330.1195945076670888No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT26850.1174940552821564No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA26690.11679390448717893No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC26390.11548112174659617No Hit
GTATCAACGCAGAGTACGGGAGGTCTGGGAGAGGAGCCCCAGCCCTGAGATTCCCAGGTGTTTCCATTCAGTGAT26340.11526232462316573No Hit
GTCTTTGTCTCCAGGGGAGAGGGCCACCCTCTCCTGCAGGGCCAGTCACT26330.11521856519847963No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT26130.11434337670475779No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25770.1127680374160585No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA25280.11062382560643998No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGACTGAGCTGGATTTTC25050.10961735883865988No Hit
CTCCAGGCCCTTCCCTGGAGCTTGCCGGACCCAGTGCATGGCAAAGTCAT24790.10847961379682149No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG24290.10629164256251691No Hit
GTCTCAGACCCACTGCCACTGAACCTGCTTGGGATGCCAGTGGCCCTCAC24060.10528517579473679No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA23600.10327224225917657No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC23290.10191570009390773No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23230.10165314354579116No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)