FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755893

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755893
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1657518
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29630.17876125628801617No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25100.15143123634253142No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19460.11740445654285503No Hit
CCCTTGGACAGGCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA17420.10509689789190828No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17160.10352828747561113No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28400.044.6746331
GTGGTAT8650.041.3399731
GTATCAA72950.040.4874881
TGGTATC8600.040.379672
TATCAAC82250.035.9084852
ATCAACG86850.034.1253623
TCAACGC87250.033.9689184
CAACGCA88900.033.4544755
AACGCAG90650.032.884496
ACGCAGA98500.030.2573597
CGCAGAG99100.029.97018
AGAGTAC99950.029.61205111
CAGAGTA102950.028.78253710
GCAGAGT104350.028.5281499
AGTACGG65850.025.94470613
GAGTACG66450.025.76217312
TATACCG1001.6060054E-524.0678835
AGTACTT53450.023.28139713
TACGGGA27250.023.08584615
GAGTACT54400.022.93801512
Produced by FastQC (version 0.11.5)