FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755894

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755894
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1657518
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27070.1633164768044751No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23300.14057162576816662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT10900.045.428411
TGGTATC11050.044.1639632
GGTATCA31900.043.3338131
GTATCAA80550.041.3239021
TATCAAC89650.036.9929542
ATCAACG96000.034.5630153
TCAACGC96550.034.2963564
CAACGCA98550.033.5503275
AACGCAG99500.033.261466
ACGCAGA107050.030.8515267
CGCAGAG107850.030.6226798
AGAGTAC107950.030.33923711
CAGAGTA110300.029.81811310
GCAGAGT112150.029.3873989
AGTACGG71000.026.71748413
GAGTACG71650.026.61150612
GAGTACT55850.024.86810312
AGTACTT55400.024.64348413
TACGGGA27550.023.41439615
GTACGGG80300.023.32696714
Produced by FastQC (version 0.11.5)