FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755896

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755896
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1424851
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACTGTTGGCAGTAATAAATTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC81560.5724107292622176No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47340.33224526634714785No Hit
GTATAAAACTTACCCGTACACTTTTGGCCAGGGGACCAAGCTGGAAATCACACGAACTGTGGCTGCACCAT46820.32859576194282775No Hit
GATCTATGCTGCCTCCATTTTGCAAAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCA42910.30115429613342026No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC35240.24732410616969774No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34160.23974436625303278No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32640.2290765841480969No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32040.2248656175277275No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31900.22388305864964125No Hit
GCATAGATCAGGGACTTAGGGGCCTTCCCTGGTTGCTGCTGAAACCAGGC28380.19917872114347396No Hit
CTTTTGGCCAGGGGACCAAGCTGGAAATCACACGAACTGTGGCTGCACCA27530.1932131850979506No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26560.18640545572835335No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26420.18542289685026717No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT26370.1850719829652364No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25920.1819137579999593No Hit
GTATCAACGCAGAGTACGGGAATCAGACCCAGTCAGGACACAGCATGGAC25870.1815628441149285No Hit
GTGCAGCCACAGTTCGTGTGATTTCCAGCTTGGTCCCCTGGCCAAAAGTG24800.1740532869752697No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24720.17349182475922043No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24480.17180743811107269No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT24380.17110561034101107No Hit
GTAATACACGGCCGTGTCCGCGGCAGTCACAGAGTTCAACTTCAGGGAAA24310.17061433090196798No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24180.16970195480088796No Hit
GGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCT24160.16956158924687564No Hit
GTCCCTGATCTATGCTGCCTCCATTTTGCAAAGTGGGGTCCCATCAAAGT23750.1666840953896232No Hit
ATAGGAGACAGAGTCAGCATCACTTGTCGGGCGAGTCAGGGCATTAGCAATTTCTTAGCCTGGTTTCAGCAGCAA23260.16324513931632148No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23060.16184148377619836No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC22820.16015709712805057No Hit
GACCCAGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGG22790.1599465487970321No Hit
GTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCC22080.15496357162959495No Hit
GTCTCCATCCTCACTGTCTGCATCTATAGGAGACAGAGTCAGCATCACTT21950.15405119552851493No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21660.15201589499533635No Hit
CAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGAAGTGACGT21560.1513140672252748No Hit
GGCCAAAAGTGTACGGGTAAGTTTTATACTGTTGGCAGTAATAAATTGCA21530.15110351889425633No Hit
GTATCAACGCAGAGTACGGGTAAGTTTTATACTGTTGGCAGTAATAAATTGCAAAATCTTCAGGCTGCAGGCTG20900.1466820039428684No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT20250.1421201234374682No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20150.14141829566740663No Hit
GCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT19390.13608440461493868No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19230.13496148018284018No Hit
AGTCAGGGCATTAGCAATTTCTTAGCCTGGTTTCAGCAGCAACCAGGGAAGGCCCCTAAGTCCCTGATCTATG19100.13404910408176013No Hit
GATCAGGGACTTAGGGGCCTTCCCTGGTTGCTGCTGAAACCAGGCTAAGA18710.13131197577852No Hit
GTCTGCATCTATAGGAGACAGAGTCAGCATCACTTGTCGGGCGAGTCAGG18560.13025923412342766No Hit
GCCACAGTTCGTGTGATTTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGG18470.12962758913037223No Hit
GTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCACT18220.1278730197052183No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17540.12310059086879963No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACTTTGATGGGACCCCA17180.12057401089657796No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTGTGATTTCCAGCTTGGTCC17180.12057401089657796No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17160.12043364534256565No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17150.12036346256555949No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAATTTATTACTGCCAACAGTATAAAACTTAC17080.11987218312651639No Hit
GGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCC16650.11685432371525163No Hit
CACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAATTTATTACTGCCAAC16640.11678414093824548No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16630.11671395816123932No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16620.11664377538423316No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16600.11650340983022085No Hit
GTTTCCCAGGTGCCAGAAGTGACGTCCAGATGACCCAGTCTCCATCCTCA16360.11481902318207307No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT16350.11474884040506692No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16340.11467865762806076No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15670.10997641156864824No Hit
GTGTATTACTGTGCGACCTCGGGGAGTGACTACTACCACGGTATGGACGT15130.10618654161031575No Hit
GTCCCAGATCCACTGCCGCTGAACTTTGATGGGACCCCACTTTGCAAAAT15040.10555489661726034No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC14830.10408105830013104No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACTTTG14740.10344941330707562No Hit
GCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGAAGTGACGTCCAGATGACCCAGTCTCCATCCTCA14610.10253703720599558No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC14280.1002210055647924No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)