FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755897

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755897
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences909501
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32940.3621766221257591No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG25040.2753158050403463No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23750.26113220326310804No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22750.250137163125714No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21400.23529385894023205No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20760.2282570332522999No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20660.2271575292385605No Hit
GTCTGTATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGG19700.21660229070666223No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAACCATTTAAATTGGTATCAGCAGAAA18270.20087938331018879No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18130.1993400776909536No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16970.18658583113157653No Hit
GTCTCCATCCTCCCTGTCTGTATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAA16290.1791092038381486No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGGTTGGTCCCCTGGCCAAAAGTG16050.17647039420517405No Hit
ATCTTCAGACTGCAGGCTGCTGATGGTGAAAGTAAAATATGTCCCAGATCCACTTCCACTGAACCTTGATGGGAC15560.17108282453785098No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15450.16987337012273762No Hit
GTCTGAAGATACTGCAACATATTACTGCCAACATTATGATAATCTCCCTATGTACACTTTTGGCCAGGGGACCAA15360.16888381651037218No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15340.1686639157076243No Hit
AGTCAGGACATTAGCAACCATTTAAATTGGTATCAGCAGAAACCAGGGAA15040.16536540366640606No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC14690.16151713961831818No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14400.1583285779784739No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG14030.1542604131276381No Hit
CTTTTGGCCAGGGGACCAACCTGGAGATCAAACGAACTGTGGCTGCACCA13040.14337532339161804No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12730.1399668609490259No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12700.13963700974490406No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12620.13875740653391255No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12590.13842755532979073No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC12250.13468924168307678No Hit
GATCTACGATGCATCCAATTTGGAAACAGGGGTCCCATCAAGGTTCAGTGGAAGTGGATCTGGGACATATTTTA12190.13402953927483313No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT12110.1331499360638416No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGGTTGGTCC11660.1282021680020143No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11650.12809221760064035No Hit
GGCCAAAAGTGTACATAGGGAGATTATCATAATGTTGGCAGTAATATGTTGCAGTATCTTCAGACTGCAGGCTG11600.12754246559377067No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11490.1263330111786573No Hit
ATCATAATGTTGGCAGTAATATGTTGCAGTATCTTCAGACTGCAGGCTGC11390.1252335071649179No Hit
ATGTACACTTTTGGCCAGGGGACCAACCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG11160.12270464793331728No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT11060.12160514391957788No Hit
GGACATTAGCAACCATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTACGATGCATC10820.11896633428660332No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10700.11764692947011603No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA10700.11764692947011603No Hit
GTAATATGTTGCAGTATCTTCAGACTGCAGGCTGCTGATGGTGAAAGTAAAATATGTCCCAGATCCACTTCCA10620.11676732625912452No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10510.11555787184401116No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10490.1153379710412633No Hit
CATTATGATAATCTCCCTATGTACACTTTTGGCCAGGGGACCAACCTGGA10080.11083000458493174No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10050.11050015338080993No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT9870.10852104615607899No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9710.10676183973409593No Hit
ACATATTACTGCCAACATTATGATAATCTCCCTATGTACACTTTTGGCCA9390.10324342689012986No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT9380.10313347648875591No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9300.1022538732777644No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9290.10214392287639046No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9240.10159417086952076No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9120.10027476605303348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATT853.0930096E-853.51209370
GTGGTAT4250.046.1579061
GTATCAA31500.040.862151
CGTAAAC557.517403E-637.5447043
ACGTAAT805.7736906E-736.8792369
TATCAAC35850.035.9039762
ATCAACG38300.033.786973
TCAACGC38500.033.611454
CAACGCA39400.032.8436745
AACGCAG40450.032.0762026
CGCAGAG42850.030.2646548
CGGACGC703.5957506E-529.86907853
AGAGTAC44300.029.19479811
ACGGGAC1900.028.9707216
CAGAGTA45050.028.70875510
GCAGAGT45250.028.5818639
TGATTCG500.007365107527.87780653
ACGCAGA46650.027.7993667
GCGCGCT500.0076786127.64073238
CGAACTA500.00783295627.52824224
Produced by FastQC (version 0.11.5)