FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755902

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755902
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences256601
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5710.22252446405119233No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGA300.003542475662.22114670
GTAATAC252.6150065E-454.9777763
CACTCCG200.007476516551.5316247
GTGGTAT1750.047.215851
GTATCAA13250.042.0933761
GGTATCA5250.041.313871
TGGTATC2100.039.308152
TATCAAC15650.035.3836172
TCTAGTA400.002631766534.3946342
ATCAACG16450.033.8389053
TCAACGC16450.033.832314
CAACGCA16800.032.922985
AACGCAG17050.032.641736
CGCTAAG552.8625198E-432.04954563
ACGCTAA808.295865E-531.11057370
GTTGTCT450.00469012130.56704326
ACGCAGA18250.030.4954267
CGCAGAG18550.030.0022378
CAGAGTA19300.028.8363510
AGAGTAC19650.028.31720411
Produced by FastQC (version 0.11.5)