FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1157983
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13190.1139049536996657No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA48300.040.5870361
GTGGTAT7150.036.972861
GGTATCA20600.035.8318821
TATCAAC56250.034.972812
ATCAACG59150.033.2581633
TCAACGC59200.033.114094
CAACGCA60800.032.2991335
AACGCAG61550.032.0171176
CGTACGC450.00411427131.40563863
TGGTATC8750.030.996842
ACGCAGA66400.029.6746737
CGCAGAG67900.028.9685658
AGAGTAC69700.028.12195211
CAGAGTA71300.027.58717510
GCAGAGT72300.027.205619
TTCCGTA1158.3757914E-426.68684870
GAGTACG45000.023.49526612
AGTACGG45400.023.21264613
GAGTACT39950.022.59857612
TCGTATG1253.379624E-622.171640
Produced by FastQC (version 0.11.5)