FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755906

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755906
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1157983
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16270.14050292620876126No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT8200.044.3842851
GTATCAA57750.041.6776541
GGTATCA22650.037.139421
TGGTATC9450.037.0455862
TATCAAC66450.035.948632
TCAACGC68200.034.9275254
ATCAACG68350.034.8217853
CAACGCA69750.034.138085
AACGCAG71500.033.4448436
ACGCAGA77050.031.0357727
CGCAGAG78850.030.3707338
AGAGTAC80200.029.77406911
CAGAGTA81700.029.31128910
GCAGAGT83600.028.6041419
CGGGCTA1707.2759576E-1226.20289417
GAGTACG51350.025.7529312
AGTACGG51500.025.67903113
GAGTACT42800.024.81405312
AGTACTT43650.024.09642813
TACGGGT11600.023.62921515
Produced by FastQC (version 0.11.5)