FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences955601
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12900.1349935799564881No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTAT504.5553537E-449.14040470
GTATCAA35700.041.6506461
GGTATCA14100.041.2547341
GTGGTAT4700.038.0812951
TATCAAC42100.035.5642282
TGGTATC5200.033.7577172
ATCAACG44400.033.334333
TCAACGC44700.033.187614
CAACGCA45200.032.668195
AACGCAG46650.031.7265646
TCCGTTA550.0049698630.21480869
ACGCAGA49300.030.0117577
CGCAGAG51100.028.954598
ATTGTCG500.00636366128.72493467
AGAGTAC52450.027.9469211
CAGAGTA53000.027.7867510
GCAGAGT54250.027.0830769
AAGCGTC658.715862E-426.50716432
TCGTATG2150.025.76836440
CGTATGC2300.024.0979741
Produced by FastQC (version 0.11.5)