FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755908

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755908
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences955601
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23500.2459185371300365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42800.043.4083141
GTGGTAT5500.041.2861331
GGTATCA16450.040.365871
TATCAAC48700.038.1899952
ATCAACG51050.036.2706763
TCAACGC51400.035.8693584
CAACGCA52500.035.034075
AACGCAG53750.034.2831656
TGGTATC6300.033.832372
ACGCAGA56550.032.8284037
CGCAGAG57750.032.1462558
AGAGTAC59550.031.23220411
CAGAGTA59600.031.03327410
GCAGAGT61700.030.0326549
GAGTACG42200.026.1821812
AGTACGG42100.026.16834313
GAGTACT29450.025.39999212
CTTAACG700.001210043825.05758958
AGTACTT30200.024.31980713
TACGGGG19500.023.94259815
Produced by FastQC (version 0.11.5)