FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755909

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755909
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences837543
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50260.6000885924662972No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGGACACAGTGATGGAAACACCTACTTGA41240.49239262939335654No Hit
GTGTGTACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCC32100.3832639040622392No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31190.3723987902710667No Hit
GTACACACTGGCCTCCAACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA31140.37180180599682644No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30690.3664289475286642No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30280.3615336764798942No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29930.3573547865602124No Hit
GTGATGGAAACACCTACTTGACTTGGTTTCAGCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTT28760.34338535454299063No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28760.34338535454299063No Hit
GTCCCAGACAGATTCAGCGGCAGTGAGTCAGGCACTGATTTCACACTGAA24910.2974175654264915No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24850.2967011842974032No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24730.29526842203922665No Hit
GGTACACACTGGCCTCCAACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT24540.2929998817971137No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC23170.2766425126829309No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTTCTGACCGGGAC20960.25025580776151196No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTT20580.24571872727728608No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19320.2306747235664318No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19110.22816738961462277No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18400.21969021292041124No Hit
GTCTGGGACCCCAGAGTCCCGGTCAGAAACCTTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGAAA18360.21921262550101905No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA17850.21312338590376853No Hit
AGGTACACACTGGCCTCCAACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT17360.2072729400162141No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17170.20500439977410115No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG16840.20106430356411553No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC16230.19378109541838448No Hit
CAGTGATGGAAACACCTACTTGACTTGGTTTCAGCAGAGGCCAGGCCAAT16110.19234833316020788No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGT15030.17945347283661855No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14810.17682674202996146No Hit
TTATTACTGCATGCAAGGTACACACTGGCCTCCAACTTTTGGCCAGGGGA14520.173364233239368No Hit
GGACTCTGGGGTCCCAGACAGATTCAGCGGCAGTGAGTCAGGCACTGATT14460.1726478521102797No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14400.1719314709811914No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14160.16906594646483822No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14070.16799137477120576No Hit
AGTGTGTACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACC13990.1670361999324214No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13980.16691680307757334No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGACTCACTGCCG13970.1667974062227253No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13910.16608102509363698No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13900.16596162823878893No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACT13760.16429007227091624No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13710.16369308799667598No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13660.16309610372243574No Hit
GGTCTAGTCAAAGCCTCGGACACAGTGATGGAAACACCTACTTGACTTGGTTTCAGCAGAGGCCAGGCCAATCTC13620.16261851630304355No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13600.16237972259334743No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACTCTCC13360.15951419807699424No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13100.15640987985094496No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13090.15629048299609694No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGA12790.15270857735065543No Hit
GACCGGGACTCTGGGGTCCCAGACAGATTCAGCGGCAGTGAGTCAGGCAC12750.15223098993126322No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG12670.15127581509247884No Hit
GTCAGGCACTGATTTCACACTGAAAATCAGCAGGGTGGAGGCTGAGGATG12660.1511564182376308No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12630.15079822767308665No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12530.14960425912460615No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12470.14888787799551784No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGGACA12440.1485296874309737No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12200.1456641629146205No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12180.1454253692049244No Hit
GCCTCGGACACAGTGATGGAAACACCTACTTGACTTGGTTTCAGCAGAGG12090.14435079751129196No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11840.14136587614009072No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11590.13838095476888948No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11540.13778397049464924No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11470.13694819251071289No Hit
GTTTATTACTGCATGCAAGGTACACACTGGCCTCCAACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA11370.13575422396223238No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG11240.13420206484920774No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10980.13109774662315846No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10910.13026196863922213No Hit
GGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGGTACACACTGGCCTCCAACTTTTGGCCAGGGGA10870.1297843812198299No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10770.1285904126713494No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT10770.1285904126713494No Hit
GGCCAATCTCCAAGGCGCCTAATTTATAAGGTTTCTGACCGGGACTCTGG10670.12739644412286893No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT10490.12524730073560403No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC10350.12357574476773132No Hit
AGTCAAAGCCTCGGACACAGTGATGGAAACACCTACTTGACTTGGTTTCAGCAGAGGCCAGGCCAATCTCCAAGG10250.12238177621925084No Hit
GATTTCACACTGAAAATCAGCAGGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGGTACACACTGG10240.12226237936440278No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTC10090.12047142654168204No Hit
TTCCATCACTGTGTCCGAGGCTTTGACTAGACCTGCAGGAGATGGAGGCC9950.11879987057380934No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9870.11784469573502496No Hit
GAGTCAGGCACTGATTTCACACTGAAAATCAGCAGGGTGGAGGCTGAGGA9760.11653133033169641No Hit
GGCCTGGCCTCTGCTGAAACCAAGTCAAGTAGGTGTTTCCATCACTGTGT9650.11521796492836786No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT9540.11390459952503931No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC9490.11330761525079906No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA9440.11271063097655881No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9410.11235244041201467No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC9370.11187485299262248No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG9220.11008390016990172No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC9160.10936751904081343No Hit
GCAGTGAGTCAGGCACTGATTTCACACTGAAAATCAGCAGGGTGGAGGCT9050.10805415363748488No Hit
CTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTA8970.10709897879870049No Hit
TCTCCAAGGCGCCTAATTTATAAGGTTTCTGACCGGGACTCTGGGGTCCC8720.10411405742749924No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8670.10351707315325899No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGACTCACTGCCG8670.10351707315325899No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8590.1025618983144746No Hit
GTTTCTGACCGGGACTCTGGGGTCCCAGACAGATTCAGCGGCAGTGAGTC8450.1008903423466019No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA8440.10077094549175386No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC8430.1006515486369058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3200.053.7330631
GCGTCGT200.006986900752.432358
TGGTATC3350.052.3567732
GGTATCA10500.043.8871231
GTATCAA28400.042.5020981
CTTATAC1950.038.7980271
TATCAAC33550.035.877572
ATCAACG35350.034.245283
TCAACGC36050.033.5803264
ATCAGTG15750.033.4231770
CAACGCA36500.033.166325
AACGCAG37250.032.4985436
CGTCAGA1400.031.93475
TATACAC2450.030.8819073
CGCAGAG40850.029.6256898
ATCTCGT2000.029.34821537
AGAGTAC41700.029.02007511
ACGCAGA41900.028.8832787
CGTATGC2400.028.840241
CAGAGTA42350.028.65584410
Produced by FastQC (version 0.11.5)