FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755913

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755913
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences816068
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35050.039.2911721
GGTATCA15050.039.0209851
CGTATAC451.02856146E-438.158923
GCCGTCT1550.035.9287446
TCTCGTA1450.035.75078238
CGTATGC1900.034.65527741
CTCGTAT1600.034.5782839
TCGTATG1850.033.67698740
TATCAAC41000.033.672922
TCAACGC42250.033.0018234
ATCAACG42250.032.8392523
CAACGCA43050.032.3885465
GACTAGG2150.031.96071826
GTGGTAT5450.031.5073661
AACGCAG44050.031.4973536
CCGTCTT1950.030.3644147
ACGCAGA47550.029.31447
CGCAGAG47700.029.2204278
AGAGTAC48750.028.52064111
ACGAGAC2300.028.3651122
Produced by FastQC (version 0.11.5)