FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755916

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755916
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1758445
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27230.15485272499282035No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26520.15081506672088124No Hit
GTGTGTACCTTGCATGCAGTAATAAACCCCAGCATCCTCAGCCTCCACCC24590.13983946043237064No Hit
CCCTTGGACAGGCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA18830.10708324684593491No Hit
GTACACACTGGCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA18800.1069126415668389No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC10300.044.6240462
GTGGTAT10950.042.314891
GGTATCA31450.041.5793721
GTATCAA81250.041.3977051
TATCAAC88650.037.7248272
ATCAACG96350.034.831543
TCAACGC96950.034.6345754
CAACGCA99200.033.8700645
AACGCAG101300.033.2345966
ACGCAGA109750.030.675767
CGCAGAG110250.030.4434458
AGAGTAC110700.030.0713311
GCAGAGT112850.029.6519
CAGAGTA113000.029.6116410
CGATTTA550.00964968826.3769167
AGTACGG74700.025.30912613
GAGTACT56000.025.25782812
AGTACTT55550.025.09395213
GAGTACG76350.025.02990312
TACGGGT10400.022.40432515
Produced by FastQC (version 0.11.5)