FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755924

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755924
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences487191
Sequences flagged as poor quality0
Sequence length20-76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7680.15763838001933533No Hit
GTATCAACGCAGAGTACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTC5230.10735009472670884No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5170.10611854488280777No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8450.045.6360861
GTATCAA24650.042.741681
TATCAAC27850.037.695342
ATCAACG28850.036.224723
TCAACGC29200.035.5295034
AAGCGCG400.002416837535.003554
CAACGCA29900.034.8018845
GTATCGA400.002620541334.4307981
GTGGTAT3050.033.866361
TGGTATC2750.033.7943532
AACGCAG31100.033.6798936
ACGCAGA33250.031.50217
ACGGGTA553.3367833E-431.23002816
CGCAGAG33650.031.1276288
TACCGTA450.00452872630.79209939
AACGTCG450.00472470930.5266235
AGAGTAC35900.029.36805511
GCAGAGT35500.029.312019
CAGAGTA36350.028.81553810
TACGGGA7850.028.44226315
Produced by FastQC (version 0.11.5)