FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences384027
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAGTTACCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT26690.695003215919714No Hit
CTATTATACTGTTGGCAATAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC24550.6392779674345814No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16130.4200225504977515No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11870.30909285024230065No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11370.2960729323719426No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC11320.2947709405849068No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11000.2864381931478776No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10130.26378353605345456No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9890.25753397547568274No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9870.2570131787608684No Hit
ATAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGCATTAGCAATTATTTAGCCTGGTATCAGCAGAAA9560.24894082968124637No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8700.22654657094423047No Hit
CAGTATAATAGTTACCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT8250.21482864486090822No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG7840.2041523122072146No Hit
GTCTCCATCCTCACTGTCTGCATCTATAGGAGACAGAGTCACCATCACTT7670.19972554013129284No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG7540.19634036148499975No Hit
AGTCAGGGCATTAGCAATTATTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGTCCCTGATCTTTG7400.1926947844812995No Hit
GGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCT6980.18175805347019872No Hit
GCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT6620.17238371260354088No Hit
GTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCC6610.17212331424613375No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT6470.16847773724243348No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6420.16717574545539768No Hit
ATTATACTGTTGGCAATAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGC6250.16274897337947594No Hit
GACCCAGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGG6180.16092618487762578No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6100.15884299801836851No Hit
GTCTGCATCTATAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGG6090.15858259966096133No Hit
GTATCAACGCAGAGTACGGGAATCAGACCCAGTCAGGACACAGCATGGAC5990.15597861608688973No Hit
GTTTCCCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCA5950.15493702265726106No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG5900.15363503087022526No Hit
CAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGATGTGACAT5790.1507706489387465No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG5450.141917104786903No Hit
GTCCCTGATCTTTGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAAGT5300.13801112942579558No Hit
TCCTCACTGTCTGCATCTATAGGAGACAGAGTCACCATCACTTGTCGGGC5190.1351467474943168No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTATTGCCAACAGTATAATAGTTAC5180.13488634913690964No Hit
GGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCC5170.13462595077950248No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT5170.13462595077950248No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT5150.13410515406468818No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG5070.13202196720543088No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA4970.12941798363135926No Hit
CACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTATTGCCAACAGTATAATAGTTACCCTCTCACTTT4910.12785559348691627No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA4910.12785559348691627No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA4790.12473081319803035No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA4650.12108523619433009No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT4650.12108523619433009No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC4530.11796045590544416No Hit
GCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCA4480.11665846411840836No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA4460.116137667403594No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA4440.1156168706887797No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG4420.11509607397396537No Hit
CTATAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGCATTAGC4410.1148356756165582No Hit
CCATCCTCACTGTCTGCATCTATAGGAGACAGAGTCACCATCACTTGTCG4240.11040890354063647No Hit
GATCAGGGACTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCTAAAT4210.10962770846841498No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC4160.10832571668137918No Hit
GTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCACT4150.10806531832397201No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAGTCCCTGATCTTTGCTGCATCCAGTTTGCAAAGTGGGGTCCCAT4100.10676332653693621No Hit
GCCCCTAAGTCCCTGATCTTTGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGAT4090.10650292817952903No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA4090.10650292817952903No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC3990.10389894460545743No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC3980.10363854624805026No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG3960.10311774953323594No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT3880.10103456267397866No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGTG200.00674298852.8998664
GTGGTAT1400.051.4528121
GTATCAA12400.043.1539731
TATCAAC14600.036.886262
TGTTAGA3300.034.4221570
ATCAACG15650.034.411473
TCAACGC15650.034.411474
TATTAGA400.00266849534.3018762
GTATTAG501.9231066E-434.3018721
CCGTGAT1002.2411166E-433.40973370
CAACGCA16400.033.2560845
AACGCAG16750.032.5611846
AATCTGT7550.031.25889468
ACGGGTG450.004750864630.4905595
ACGGGTA450.004753893230.48658615
CGCAGAG17950.030.18158
GTCCAGA802.8818322E-630.0141411
ACGCAGA18350.029.7104517
AGAGTAC18250.029.68536611
GTCAGAC952.7132592E-729.59906862
Produced by FastQC (version 0.11.5)