FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1104040
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12350.11186188906199052No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA48450.039.822951
GGTATCA20950.038.346231
GTGGTAT6600.035.891861
TCGTATG2750.035.2864440
TATCAAC55500.034.8262142
ATCAACG57350.033.5231973
TCAACGC58050.033.059824
CGTATGC2950.032.93329241
CAACGCA59050.032.5580945
AACGCAG60300.031.7693066
CGTGTAT450.004711719730.54719427
ACGAGAC3500.030.41189822
CTTATAC3550.029.9794881
CTCGTAT2900.029.84475339
ACGCAGA64800.029.5564127
CGCAGAG66050.029.049028
TGGTATC8450.028.0338822
TCTCGTA3100.027.89507138
CAGAGTA68950.027.87701210
AGAGTAC69150.027.84602411
Produced by FastQC (version 0.11.5)