FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755935

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755935
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1029072
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT52400.509196635415209No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32620.31698462303900987No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31820.3092106286051899No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31050.30172815896263816No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29560.28724909432964846No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29430.2859858202341527No Hit
GTACTTAACTAATCCTCCGACGTTCGGCCAAGGGTCCAAGGTGGAAATCGAACGAACTGTGGCTGCACCAT27070.2630525366543838No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25920.25187741965576754No Hit
GTTCTATGCTGCATTTACATTGCAAAGTGGGGTCCCATCCAGGTTCAGTGGCAGTGGATCTGGGACGGATTACA25630.2490593466735078No Hit
ATAGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGGGCCTTAGTCATGCTTTAGCCTGGTATCAGCAGAAA24430.23739835502277779No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24140.23458028204051806No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22900.22253059066809708No Hit
GGATTACACTCTCACTATCAGCAGCCTGCAGCCTGAAGATTTTGCGACTT22880.2223362408072516No Hit
TGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC22710.22068426699006485No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22070.21446507144300886No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21910.21291027255624487No Hit
CTACAGTACTTAACTAATCCTCCGACGTTCGGCCAAGGGTCCAAGGTGGA21550.20941197506102585No Hit
GTTAAGTACTGTAGACAGTAATAAGTCGCAAAATCTTCAGGCTGCAGGCTGCTGATAGTGAGAGTGTAATCCGTC21280.2067882519396116No Hit
GATTACACTCTCACTATCAGCAGCCTGCAGCCTGAAGATTTTGCGACTTATTACTGTCTACAGTACTTAACTAAT20930.2033871293748154No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20740.20154080569678312No Hit
GTCTCCATCCTCCCTGTCTGCATCTATAGGAGACAGAGTCACCATCACTT20020.19454421070634514No Hit
ACTTATTACTGTCTACAGTACTTAACTAATCCTCCGACGTTCGGCCAAGGGTCCAAGGTGGAAATCGAACGAA19840.19279506195873564No Hit
GTGCAGCCACAGTTCGTTCGATTTCCACCTTGGACCCTTGGCCGAACGTC19800.19240636223704463No Hit
GTACTGTAGACAGTAATAAGTCGCAAAATCTTCAGGCTGCAGGCTGCTGA19530.1897826391156304No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGCACCAGATGTGACAT19000.18463236780322465No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTCGATTTCCACCTTGGACC18180.17666402350855917No Hit
GTCTACAGTACTTAACTAATCCTCCGACGTTCGGCCAAGGGTCCAAGGTGGAAATCGAACGAACTGTGGCTGCAC17880.17374877559587668No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC17210.16723805525755245No Hit
GTGGGGTCCCATCCAGGTTCAGTGGCAGTGGATCTGGGACGGATTACACT16840.1636425828319107No Hit
GCATTTACATTGCAAAGTGGGGTCCCATCCAGGTTCAGTGGCAGTGGATC16390.15926971096288695No Hit
GTAATAAGTCGCAAAATCTTCAGGCTGCAGGCTGCTGATAGTGAGAGTGTAATCCGTCCCAGATCCACTGCCA16230.15771491207612295No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15900.15450813937217223No Hit
ACTGTAGACAGTAATAAGTCGCAAAATCTTCAGGCTGCAGGCTGCTGATAGTGAGAGTGTAATCCGTCCCAGATC15780.1533420402070992No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15490.1505239672248395No Hit
AGTCAGGGCCTTAGTCATGCTTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGTTCTATG14880.14459629646905173No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14880.14459629646905173No Hit
GCTTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGTT14710.142944322651865No Hit
GTGTAATCCGTCCCAGATCCACTGCCACTGAACCTGGATGGGACCCCACT14460.14051494939129625No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14320.13915450036537774No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14240.13837710092199576No Hit
CTCACTATCAGCAGCCTGCAGCCTGAAGATTTTGCGACTTATTACTGTCT14160.13759970147861375No Hit
CACTATCAGCAGCCTGCAGCCTGAAGATTTTGCGACTTATTACTGTCTACAGTACTTAACTAATCCTCCGACGTT14040.13643360231354074No Hit
GTATCAACGCAGAGTACGGGGAGTCAGACCCTGTCAGGACACAGCATGGA13960.13565620287015875No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13850.13458727863550848No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG13710.13322682960958998No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13710.13322682960958998No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13340.12963135718394825No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13160.12788220843633877No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13050.12681328420168853No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13030.126618934340843No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12710.12350933656731503No Hit
CTATAGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGGGCCTTAGT12530.12176018781970552No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12470.12117713823716902No Hit
GAACAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCTAAAG12260.11913646469829128No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12090.11748449088110453No Hit
CCTTGGACCCTTGGCCGAACGTCGGAGGATTAGTTAAGTACTGTAGACAGTAATAAGTCGCAAAATCTTCAGG11900.1156381672030723No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11810.11476359282926753No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11680.1135003187337718No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC11550.11223704463827604No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11520.11194551984700779No Hit
GTCTGCATCTATAGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGG11500.11175116998616229No Hit
GCATAGAACAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC11390.11068224575151203No Hit
GGGCCTTAGTCATGCTTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCC11370.11048789589066653No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11210.10893309700390254No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11190.10873874714305704No Hit
GTCCCATCCAGGTTCAGTGGCAGTGGATCTGGGACGGATTACACTCTCAC11150.10835004742136603No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11040.1072811231867158No Hit
GCCACAGTTCGTTCGATTTCCACCTTGGACCCTTGGCCGAACGTCGGAGG11040.1072811231867158No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC10970.10660089867375656No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT10920.1061150240216428No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA10900.1059206741607973No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10760.1045602251348788No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC10710.10407435048276506No Hit
CTCCTGTTCTATGCTGCATTTACATTGCAAAGTGGGGTCCCATCCAGGTT10650.10349130090022857No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10510.10213085187431006No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3500.046.1060751
TACCTAG306.4765866E-445.7590687
GTATCAA33850.042.2966271
TATCAAC37950.037.4556162
ATCAACG40800.034.839233
TCAACGC41150.034.3760344
CAACGCA42550.033.1642845
AACGCAG43400.032.5147556
CGCAGAG46750.030.245038
ACGCAGA47700.029.6426667
AGAGTAC48300.029.55865311
TCGTATG605.341383E-428.82349640
CAGAGTA49550.028.6744510
CGCATCC605.511975E-428.66906530
TACCGCG605.590989E-428.5994178
GCAGAGT49700.028.5188529
CGAGGGT600.007055162528.12421269
TGTTAGA8700.027.89673470
GCCGTCG7250.027.30943770
GAGTACT28900.026.24416712
Produced by FastQC (version 0.11.5)