FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755936

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755936
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1029072
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50640.49209384766080505No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28290.27490787816595924No Hit
GTACTTAACTAATCCTCCGACGTTCGGCCAAGGGTCCAAGGTGGAAATCGAACGAACTGTGGCTGCACCAT28270.2747135283051137No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26610.25858248985493726No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26510.25761074055070976No Hit
GTTCTATGCTGCATTTACATTGCAAAGTGGGGTCCCATCCAGGTTCAGTGGCAGTGGATCTGGGACGGATTACA26130.25391809319464526No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25660.24935087146477605No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23030.22379386476359284No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22700.22058709205964205No Hit
CTACAGTACTTAACTAATCCTCCGACGTTCGGCCAAGGGTCCAAGGTGGA22260.2163113951210411No Hit
TGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC21510.20902327533933485No Hit
ATAGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGGGCCTTAGTCATGCTTTAGCCTGGTATCAGCAGAAA20860.20270690486185614No Hit
GGATTACACTCTCACTATCAGCAGCCTGCAGCCTGAAGATTTTGCGACTT20770.20183233048805138No Hit
GATTACACTCTCACTATCAGCAGCCTGCAGCCTGAAGATTTTGCGACTTATTACTGTCTACAGTACTTAACTAAT19920.19357246140211765No Hit
ACTTATTACTGTCTACAGTACTTAACTAATCCTCCGACGTTCGGCCAAGGGTCCAAGGTGGAAATCGAACGAA19590.1903656886981669No Hit
GTTAAGTACTGTAGACAGTAATAAGTCGCAAAATCTTCAGGCTGCAGGCTGCTGATAGTGAGAGTGTAATCCGTC19470.1891995895330939No Hit
GTCTCCATCCTCCCTGTCTGCATCTATAGGAGACAGAGTCACCATCACTT19460.18910241460267113No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19390.1884221900897119No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18830.1829803939860379No Hit
GTGCAGCCACAGTTCGTTCGATTTCCACCTTGGACCCTTGGCCGAACGTC18820.18288321905561516No Hit
GTACTGTAGACAGTAATAAGTCGCAAAATCTTCAGGCTGCAGGCTGCTGA18470.17948209649081892No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGCACCAGATGTGACAT18170.1765668485781364No Hit
GTGGGGTCCCATCCAGGTTCAGTGGCAGTGGATCTGGGACGGATTACACT18090.17578944913475442No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18000.17491487476094966No Hit
GTCTACAGTACTTAACTAATCCTCCGACGTTCGGCCAAGGGTCCAAGGTGGAAATCGAACGAACTGTGGCTGCAC17550.17054200289192595No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC17070.16587760623163395No Hit
GTATCAACGCAGAGTACGGGGAGTCAGACCCTGTCAGGACACAGCATGGA16430.15965841068457795No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16370.15907536110204146No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTCGATTTCCACCTTGGACC16100.15645163798062722No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16030.15577141346766796No Hit
GCTTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGTT15850.15402226472005845No Hit
GTAATAAGTCGCAAAATCTTCAGGCTGCAGGCTGCTGATAGTGAGAGTGTAATCCGTCCCAGATCCACTGCCA15680.1523702909028717No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15630.15188441625075796No Hit
ACTGTAGACAGTAATAAGTCGCAAAATCTTCAGGCTGCAGGCTGCTGATAGTGAGAGTGTAATCCGTCCCAGATC15250.1481917688946935No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14820.14401324688651523No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14700.14284714772144222No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14510.14100082404341No Hit
CTCACTATCAGCAGCCTGCAGCCTGAAGATTTTGCGACTTATTACTGTCT14490.14080647418256448No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14380.13973754994791424No Hit
GTGTAATCCGTCCCAGATCCACTGCCACTGAACCTGGATGGGACCCCACT14350.13944602515664598No Hit
GCATTTACATTGCAAAGTGGGGTCCCATCCAGGTTCAGTGGCAGTGGATC14120.13721100175692275No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13920.13526750314846775No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13640.13254660509663074No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13360.12982570704479376No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13260.12885395774056627No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13110.12739633378422502No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG12690.12331498670646952No Hit
CACTATCAGCAGCCTGCAGCCTGAAGATTTTGCGACTTATTACTGTCTACAGTACTTAACTAATCCTCCGACGTT12540.12185736275012826No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12290.11942798948955953No Hit
AGTCAGGGCCTTAGTCATGCTTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGTTCTATG12280.11933081455913677No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT12160.11816471539406379No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12080.11738731595068179No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11610.11282009422081253No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC11560.1123342195686988No Hit
CTCCTGTTCTATGCTGCATTTACATTGCAAAGTGGGGTCCCATCCAGGTT11550.11223704463827604No Hit
CCTTGGACCCTTGGCCGAACGTCGGAGGATTAGTTAAGTACTGTAGACAGTAATAAGTCGCAAAATCTTCAGG11040.1072811231867158No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11040.1072811231867158No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10980.1066980736041793No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10880.1057263242999518No Hit
CTATAGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGGGCCTTAGT10670.10368565076107406No Hit
TTATTACTGTCTACAGTACTTAACTAATCCTCCGACGTTCGGCCAAGGGT10610.10310260117853756No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC10570.10271390145684656No Hit
GTCCCATCCAGGTTCAGTGGCAGTGGATCTGGGACGGATTACACTCTCAC10480.1018393270830418No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA10330.10038170312670057No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)