FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences853143
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGAAGACTGTGCAACTTATTACTGTCAACAGAGTTACAGTACCTTGAG46550.5456295134578846No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45040.5279302531931926No Hit
GTCTTCAGGTTGCAGACCGCTGATGGTGAGAGTGAAATCTGTCTCAGATCCACTGCCACTGAACCTTGATGGGAC34640.4060280632906793No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29490.3456630365601077No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28270.33136297197538983No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28190.33042526282229356No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28010.328315417227827No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27580.32327523052993457No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24320.28506358254126213No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG23300.2731077908392848No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23280.2728733635510108No Hit
ACTTATTACTGTCAACAGAGTTACAGTACCTTGAGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA22010.2579872307456077No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATTAGTAACTTTTTAAATTGGTATCAGCAGAAA21490.25189212125048205No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC21120.24755521641741185No Hit
GAGTTACAGTACCTTGAGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT20980.24591422539949342No Hit
GATCTATGCTGCATCCGCTTTGCAGAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGAGACAGATTTCA20360.23864697946299743No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18410.21579031885627614No Hit
ACCTTGAGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC17420.20418616808670995No Hit
GTCTCCACCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATTAGTAA17180.20137304062742117No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16860.19762220401503613No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG16860.19762220401503613No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16670.19539514477643255No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16490.19328529918196596No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15880.18613526688960702No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCTCAAGGTA15630.1832049257861812No Hit
GTTACAGTACCTTGAGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA15190.1780475254441518No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT15170.17781309815587773No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15160.1776958845117407No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14980.17558603891727412No Hit
GTAATAAGTTGCACAGTCTTCAGGTTGCAGACCGCTGATGGTGAGAGTGAAATCTGTCTCAGATCCACTGCCA14820.1737106206110816No Hit
GTACTGTAACTCTGTTGACAGTAATAAGTTGCACAGTCTTCAGGTTGCAGACCGCTGATGGTGAGAGTGAAAT14760.17300733874625943No Hit
ATGCTGCATCCGCTTTGCAGAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGAGACAGATTTCACTCTCA14670.17195241594902613No Hit
CACCATCAGCGGTCTGCAACCTGAAGACTGTGCAACTTATTACTGTCAACAGAGTTACAGTACCTTGAGGACGTT14060.16480238365666716No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13670.1602310515353229No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13610.15952776967050072No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13420.1573007104318971No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13410.15718349678776009No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13390.15694906949948603No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13370.15671464221121195No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13070.153198232887101No Hit
ACCTGAAGACTGTGCAACTTATTACTGTCAACAGAGTTACAGTACCTTGA12960.15190888280159365No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC12950.15179166915745662No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC12850.15061953271608627No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12750.14944739627471595No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12570.1473375506802494No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12390.1452277050857828No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA12340.14464163686509762No Hit
CTGTAACTCTGTTGACAGTAATAAGTTGCACAGTCTTCAGGTTGCAGACCGCTGATGGTGAGAGTGAAAT12240.14346950042372733No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT11920.1397186638113423No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT11820.13854652736997197No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11550.13538175897827212No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11540.13526454533413507No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC11530.13514733168999804No Hit
GAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGAGACAGATTTCACTCTCACCATCAGCGGTCTGCAAC11320.13268584516312038No Hit
ACAGTACCTTGAGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA11160.13081042685692787No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11150.13069321321279082No Hit
CTCCTGATCTATGCTGCATCCGCTTTGCAGAGTGGGGTCCCATCAAGGTT11020.1291694358390094No Hit
GTTCAGTGGCAGTGGATCTGAGACAGATTTCACTCTCACCATCAGCGGTC10950.12834894033005018No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10940.12823172668591315No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10860.12729401753281688No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10820.12682516295626875No Hit
GTCTGCAACCTGAAGACTGTGCAACTTATTACTGTCAACAGAGTTACAGTACCTTGAGGACGTTCGGCCAAGGGA10810.12670794931213175No Hit
GAGTATTAGTAACTTTTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATC10750.12600466744730954No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10750.12600466744730954No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCACCCTCC10410.12201940354665045No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10380.12166776261423935No Hit
CCTCAAGGTACTGTAACTCTGTTGACAGTAATAAGTTGCACAGTCTTCAG10370.12155054897010231No Hit
ATCTGAGACAGATTTCACTCTCACCATCAGCGGTCTGCAACCTGAAGACT10340.12119890803769123No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCACCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCA10310.12084726710528011No Hit
GATTTCACTCTCACCATCAGCGGTCTGCAACCTGAAGACTGTGCAACTTATTACTGTCAACAGAGTTACAGTAC10290.12061283981700606No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC10220.11979234430804685No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10080.11815135329012838No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGAGACAGATTTCACT10070.11803413964599137No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT9920.11627593498393586No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9500.11135296193018052No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA9370.10982918455639909No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9290.10889147540330284No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9230.10818819353848065No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC9230.10818819353848065No Hit
CATCAAGGTTCAGTGGCAGTGGATCTGAGACAGATTTCACTCTCACCATCAGCGGTCTGCAACCTGAAGACTGTG9210.1079537662502066No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG9190.10771933896193253No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC9170.10748491167365846No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC9020.10572670701160297No Hit
AGGTACTGTAACTCTGTTGACAGTAATAAGTTGCACAGTCTTCAGGTTGCAGACCGCTGATGGTGAGAGTGAAAT8930.10467178421436969No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8770.10279636590817719No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT8750.1025619386199031No Hit
TTGCAGAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGAGACAGATTTCACTCTCACCATCAGCGGTCTG8700.10197587039921796No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAA8650.1013898021785328No Hit
GCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATCCGCTTTGCAGAGTGGGGTCCCATCAAGGTT8650.1013898021785328No Hit
GTGCAACTTATTACTGTCAACAGAGTTACAGTACCTTGAGGACGTTCGGC8570.10045209302543653No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)