FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences853143
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42170.4942899373258645No Hit
CCTGAAGACTGTGCAACTTATTACTGTCAACAGAGTTACAGTACCTTGAG42020.492531732663809No Hit
GTCTTCAGGTTGCAGACCGCTGATGGTGAGAGTGAAATCTGTCTCAGATCCACTGCCACTGAACCTTGATGGGAC35990.42185190524917865No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30070.3524614279200556No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27990.3280809899395529No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25650.3006529972114874No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24490.2870562144915917No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24300.2848291552529881No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22090.25892493989870397No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG21330.2500167029442895No Hit
GATCTATGCTGCATCCGCTTTGCAGAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGAGACAGATTTCA20980.24591422539949342No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC20590.24134289327814915No Hit
ACTTATTACTGTCAACAGAGTTACAGTACCTTGAGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA20060.23513057013888647No Hit
GAGTTACAGTACCTTGAGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT19830.23243465632373472No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19440.22786332420239044No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18630.21836901902729083No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATTAGTAACTTTTTAAATTGGTATCAGCAGAAA18490.2167280280093724No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18450.2162591734328243No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17190.20149025427155823No Hit
ACCTTGAGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC16050.18812789883993658No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15880.18613526688960702No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15210.17828195273242586No Hit
GTCTCCACCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATTAGTAA15160.1776958845117407No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT14650.17171798866075205No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14590.17101470679592987No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14570.17078027950765579No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC14400.16878764755732628No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG14360.16831879298077815No Hit
GTACTGTAACTCTGTTGACAGTAATAAGTTGCACAGTCTTCAGGTTGCAGACCGCTGATGGTGAGAGTGAAAT13990.16398188814770795No Hit
GTAATAAGTTGCACAGTCTTCAGGTTGCAGACCGCTGATGGTGAGAGTGAAATCTGTCTCAGATCCACTGCCA13980.16386467450357092No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13970.1637474608594339No Hit
ATGCTGCATCCGCTTTGCAGAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGAGACAGATTTCACTCTCA13830.16210646984151542No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13750.16116876068841918No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13740.16105154704428215No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCTCAAGGTA13640.1598794106029118No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13560.15894170144981556No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT13550.15882448780567854No Hit
GTTACAGTACCTTGAGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA13400.15706628314362303No Hit
CACCATCAGCGGTCTGCAACCTGAAGACTGTGCAACTTATTACTGTCAACAGAGTTACAGTACCTTGAGGACGTT13400.15706628314362303No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13290.15577693305811569No Hit
CTCCTGATCTATGCTGCATCCGCTTTGCAGAGTGGGGTCCCATCAAGGTT12750.14944739627471595No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12490.14639984152715313No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12100.14182850940580888No Hit
CTGTAACTCTGTTGACAGTAATAAGTTGCACAGTCTTCAGGTTGCAGACCGCTGATGGTGAGAGTGAAAT11860.1390153819465201No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11840.13878095465824603No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC11650.1365538954196424No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGAGACAGATTTCACT11640.1364366817755054No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11550.13538175897827212No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA11370.13327191338380553No Hit
ACCTGAAGACTGTGCAACTTATTACTGTCAACAGAGTTACAGTACCTTGA11360.1331546997396685No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11070.12975550405969458No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10960.1284661539741872No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC10830.1269423766004058No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10780.12635630837972062No Hit
GAGTATTAGTAACTTTTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATC10500.12307432634388374No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10350.12131612168182825No Hit
ACAGTACCTTGAGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA10260.12026119888459497No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT10150.11897184879908759No Hit
GTCTGCAACCTGAAGACTGTGCAACTTATTACTGTCAACAGAGTTACAGTACCTTGAGGACGTTCGGCCAAGGGA10070.11803413964599137No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC9960.116744789560484No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9900.11604150769566181No Hit
GATTTCACTCTCACCATCAGCGGTCTGCAACCTGAAGACTGTGCAACTTATTACTGTCAACAGAGTTACAGTAC9810.11498658489842851No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9770.11451773032188038No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT9750.11428330303360633No Hit
GTTCAGTGGCAGTGGATCTGAGACAGATTTCACTCTCACCATCAGCGGTC9610.11264231201568786No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9600.11252509837155085No Hit
GAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGAGACAGATTTCACTCTCACCATCAGCGGTCTGCAAC9390.11006361184467317No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA9380.10994639820053613No Hit
ATCTGAGACAGATTTCACTCTCACCATCAGCGGTCTGCAACCTGAAGACT9100.10666441616469922No Hit
CCTCAAGGTACTGTAACTCTGTTGACAGTAATAAGTTGCACAGTCTTCAG9090.10654720252056221No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC8930.10467178421436969No Hit
CTGATGGTGAGAGTGAAATCTGTCTCAGATCCACTGCCACTGAACCTTGA8890.10420292963782157No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC8840.1036168614171364No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCACCCTCC8640.10127258853439576No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCACCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCA8610.10092094760198467No Hit
GCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATCCGCTTTGCAGAGTGGGGTCCCATCAAGGTT8600.10080373395784763No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)